NHS Participation Drug-containing Serum On The Role Of Human Hepatoma Cell HepG2 Mechanism

1.PurposeThe use of serum pharmacology method to study drug-containing serum NHS Participation of human HepG2 hepatoma cell proliferation and apoptosis of the situation, and to explore its mechanism.2. MethodNHS Capsule will be made of the water decoction of native medicine, according to a certain dose to the rabbit gavage. The rabbits were divided into drug-containing serum group and blank control group, according to serum pharmacology method, serum was diluted into different concentrations (10%,20%), and with its cultured human liver cancer cell HepG2, and then use the MTT assay, flow Measuring-type cells, serum containing different concentrations of NHS on hepatoma cell proliferation and apoptosis effects.3.resultsâ‘ Participation NHS drug-containing serum on liver cancer cell HepG2 proliferation in vitro24h,10% and 20% NHS containing serum OD value of blank control group were significantly different, the concentration of serum containing two pairs of HepG2 inhibition rate of 11.53% and 12.49%,20% NHS containing serum and blank serum group differences were statistically significant. 48h 10% and 20% NHS containing serum OD value of blank control group were significantly different, the concentration of serum containing two pairs of HepG2 inhibition rate of 16.43% and 18.17%,20% NHS containing serum and blank serum group differences were statistically significant.â‘¡NHS Participation drug-containing serum on apoptosis of HepG2 hepatoma cellsThe results from the flow cytometry analysis point of view, found that 10% and 20% of the drug-containing serum can promote apoptosis of human hepatoma cells, and blank control group there are differences, with the blank serum group there are differences.4.Conclusionâ‘ Participation NHS containing serum can inhibit the proliferation of HepG2 hepatoma cells.â‘¡NHS containing serum parameters can induce apoptosis in HepG2 hepatoma cells.â‘¢NHS containing serum inhibited the proliferation of HepG2 hepatoma cells, induced apoptosis in HepG2 hepatoma cells and the serum concentration and culture time-dependent. Rabbit serum containing the higher the concentration, the longer the incubation time,the higher inhibition rate on cell apoptosis was more obvious.