The Study About The Proliferation On Effect Of Wugong Sanqi To Cancer Cell

Objective:To investigate the effect of Wugong Sanqi extract against tumor cells in vitro.Methods:Grouping.we set to set control group and 4 experimental groups, they are:A, B, C, D. The growth inhibitory rate of ezch group was measured by MTT assay. The cells in the logarithmic phase were inoculated in the plate with 96 wells, and inoculate for 24h, each group of different concentrationgs was set in 6 wells and reapeated 3 times, treating on Siha,HEL,Hep3B,HepG2 cell for 48h, then test the growth inbiton ratios. After each group treating on siha cells for 48h, the variations of morphology woule be observed in culture medium by nverted microscope. The cell cycle and apoptosis of Siha cells were detected by flow cytometry (FCM).Results:With the time and doses of Wugong sanqi incubation extended,wugongsanqi significantly inhibited the proliferation of siha cells and the inhibited effect shows dose-and time dependent. obvious changes of apoptotic morphology were observed by invert microscope,fluorescence microscope, scanning electron microscope and transmission electrion microscope, they showed thed cells had marked nuclear condensation or the fragmentation of chromation as well as apoptotic,mitochondrial edema and vesicle,with the time of incubation extended, the proliferation rate become slow and the volume became small and transformed. FCM assay indicated that most of the cells were arrestded in G0/G1 and the apoptotic peak appeared. During the prolong of incubationg time,the apoptosis rate was increased.Conclusion:Wugong Sanqi possers induce siha cells apoptosis in vitro.