Experimental Research About Esophageal Ning Treat Reflux Esophagitis

Objective:Esophageal ning right through the experimental observation of reflux esophagitis in rats before and after treatment histopathological changes in esophagus and PCNA (Proliferating cell nuclear antigen, PCNA) influence, thus research on esophageal ning about reflux esophagitis the role of inflammation and mechanisms of action, to explore its modulating reflux esophagitis scientific basis.Methods:Step one:To make the model and therapy:8-week-old male SD rats of clean grade, total of 40, weight (200±20) g, were randomly divided into four groups of 10 rats. Take a group as the control group (A group), normal feeding for 7 days.7 days remaining three groups according to literature and improved modeling, doing "incomplete pyloric ligation + incision of lower esophageal sphincter," after a week for delivery. Control group fed with normal saline 14 days; the other three groups were known as the model control group (B group), esophagus Ning Tang treated group (C group), omeprazole treatment group (D group), continue feeding 7 days for delivery after group. B Group with normal saline 2ml gavage, once daily; C group with Omeprazole suspension 2ml gavage, Once daily (per ml containing crude drug 0.36mg); D Group with esophageal Ning Tang solution 2ml gavage, once daily (per ml containing crude drug 1.2g), a total of gavages for 14 days. Rats before and after treatment to observe the general condition, body weight and food intake.Step two:To detect the indicatives:Visual observation of the performance of esophageal mucosa: treatment of 14 days in each group was decapitated, from gastro-esophageal junction to the throat on 0.5mc Department interception 2cm esophagus, for the naked eye.Light microscope observation of esophageal tissue pathological changes:after to the naked eye, a fixed with 10% formaldehyde, slices were doing light microscope. Observe the rat Esophageal Ning about esophagus organizational structure of the model before and after treatment effects.PCNA immunohistochemical examination:was observed by immunohistochemistry esophageal ning on rat model of esophageal PCNA before and after treatment effects.Results:1 The general condition:The activities of the control group rats were sensitive, shiny coat, eating normally. After modeling the rats in each group have different levels of activity due to not agility, movement slow, hair loose, glossy weakened, dull, eating less, abdominal distension, large toilet thin, weight stagnation, or even reduce. Esophageal Ning group and the group given omeprazole treatment, the symptoms and signs gradually improved, but the recovery rate faster than the Western Medicines Group. Finally esophagus Ning Group, Omeprazole rats were restored gradually become normal.2.The indicators of the test results:2.1 The visual observation of esophageal mucosa points:Visual observation points in model group was significantly higher than blank control group, significant difference (P<0.01). After delivery 14 days, the esophageal Ning Tang and omeprazole group decreased significantly compared with model group were significantly difference (P<0.01), compared with the blank control group had no difference (p>0.05). Esophageal Ning Tang group did not differ significantly compared with the omeprazole group (P>0.05). Tip of esophageal Ning can improve reflux esophagitis in the esophageal mucosa of rats.2.2 The optical microscope observation of esophageal mucosa points:Pathological score in model group was significantly higher than blank control group, significant difference (P<0.01). Esophageal Ning Tang group and the omeprazole group compared with the model, in the administration significantly reduced after 14 days, were significantly difference (P<0.01). Esophageal Ning Tang group and the omeprazole group and blank control group, no difference in the administration of 14 days (p>0.05). Esophageal Ning group and medicine control group did not differ significantly (P>0.05). Tip of esophageal Ning may improve the organizational structure of esophageal mucosa, so it effects RE。2.3 Esophageal mucosal PCNA immunohistochemical staining of the gray value:Model group, PCNA immunohistochemical staining gray value was significantly higher than blank control group, significant difference (P<0.01),Esophageal Ning Tang group and omeprazole group, after 14 days the administration of its PCNA immunohistochemical staining gray value significantly lower compared with the model, there are significant differences (p<0.01); with the blank control group, the two are still relatively high when compared to omeprazole group were still significantly different (p<0.05), but the group with esophageal Ning Tang was no significant difference (p>.05). Esophageal Ning Tang group and omeprazole group significantly different compared (p>0.05). Tip of esophageal Ning may inhibit increasing of PCNA expression in esophageal mucosa. so it effect RE.Conclusion:Esophageal Ning treatment of reflux esophagitis have good efficiency, can improve the model of the pathological condition of rat esophageal mucosa, inhibit PCNA expression in esophageal mucosa increased, indicating that PCNA in reflux esophagitis in rats play an important role.