Inhibitory Effect Of Cxcr4-targeted Small Interfering RNA Mediated By Liposome On Mice Model Of Laryngeal Carcinoma

Objective:To investigate the effect of CXCR4-targeted small interfering RNA(siRNA) mediated by liposome vector in inhibiting the growth of human laryngeal carcinoma cell Hep-2 xenografts in nude mice.Methods: The siRNA targeting CXCR4 mRNA was transfected into Hep-2 cells via liposome Lipofectin.Semi-quantitative RT-PCR were performed to evaluated the levels of CXCR4 gene expression. Stable monoclonal cells were selected and respectively inoculated into subcutaneous tissue of nude mice as experimental and controls.CXCR4 protein expression of tumors was investigated by immunohistochemistry. The growth of xenograft tumors was observed by measurement of tumor volume. Results:Compared to controls,the expression of tumor volume decreased in transfected group of siRNA-CXCR4.The expression of CXCR4 mRNA and protein was obviously reduced. Results:Compared to controls,the expression of tumor volume decreased in transfected group of siRNA-CXCR4.The expression of CXCR4 mRNA and protein was obviously reduced.Conlusion: CXCR4 siRNA targeting CXCR4 Mrna can markedly inhibit the growth of laryngeal carcinoma cell xenografts in nude mice through lastingly inhibition effect on CXCR4.