Molecular Recognition Of Supramolecular Host On Some Pharmaceutical Compounds

Supramolecular host which was explored the laws of host-guest molecular interactions and functional characteristics of groups, has become a new research hot topic in chemistry, biology and pharmaceutical fields in recent years. The main idea of this thesis is to improve the performance of spectroscopy and sensitizer through packing and recognizing different supramolecular host molecules with some drugs. The drug derivatives or inclusion of new species was received high fluorescence quantum yield. At the same time, new fluorescence probe systems were allowed some non-fluorescnt drugs to achieve fluorescence spectrometry. Quantitative analysis of fluorescence probe to determinate pharmaceutical preparations and the high sensitivity of drugs in biological fluids needed to be established and to achieve some of the non-fluorescent drug pharmacokinetics. The main contents of the thesis are as follows:Chapter 1: The development of supramolecule was reviewed. The cyclodextrins and cucurbituril were discussed in detail. The applications of fluorescence analysis and fluorescence probes were summarized.Chapter 2: The inclusion interactions ofβ-Cyclodextrins and several modifiedβ-Cyclodextrins with palmatine hydrochloride were studied in aqueous solution at room temperature by spectrofluorimetry. The result showed that SBE-β-CD reacted with PAL to form inclusion complexes and 1:1 stoichiometry for SBE-β-CDs-PAL complexes was established. At the same time, its association constant has been determined from fluorescence data by Benesi-Hildebrand's method (double reciprocal plots). Based on inclusion reaction, a spectrofluorimetric method with high sensitivity and selectivity was developed for the determination of PAL in aqueous solution in the presence of SBE-β-CD. The proposed methods were applied successfully for the determination of the studied drugs in tablets, urine and serum.Chapter 3: An optical probe for L-Cystine, based on the fluorescence quenching of palmatine/ cucurbit[7]urils complex immobilized has been developed. The decrease of fluorescence intensity of palmatine / cucurbit[7]urils complex upon the addition of L-Cystine was attributed to the formation of an inclusion complex between cucurbit[7]urils and L-Cystine. The probe can be applied to the quantification of L-Cystine. The probe exhibits excellent reproducibility, reversibility and selectivity. The recommended method was successfully used for the determination of L-Cystine in tablets. It also discussed the mechanism of competed reaction of palmatine as fluorescent probe and L-Cystine.Chapter 4: BRH-CB[7] inclusion complex could form strong fluorescence. Penicillamine in acidic medium could make the intensity of fluorescence in Berberine - Cucurbit[7]urils inclusion complex dramatic quenching. Based on this fact, BRH-CB[7] was used as a fluorescent probe to establish a new method of fluorescence spectroscopy of determinating penicillamine. The recommended method was successfully used for the determination of penicillame in tablets. It also discussed the mechanism of competed reaction of palmatine as fluorescent probe and penicillame.