Supramolecular Interaction Of Cucurbit[n]uril With Drug Molecules And Its Analytical Application

With the rapid development of modern pharmaceutical science,supramolecular chemistry in recent years, molecular switches,molecular catalysis and drug delivery and other fields has beenwidely studied, this paper aims to form supramolecular inclusioncomplexes, to achieve some improve the spectral properties of theguest molecule drugs. The establishment of high-sensitivity analysisof the new method of drug to achieve certain drugs trace inpharmaceutical preparations and biological fluids analysis. Thecontent of this paper are as follows:1. Introduced a new subject Cucurbituril supramolecular structurecharacteristics and applications, describes the main elements ofMolecular Catalysis Research molecular assembly, and in theanalysis of drugs in; reviewed Cucurbituril interaction with variousguest molecules between research and guest molecules to the bodycavity competition cucurbituril response status quo. 2. In this work, the competitive interaction between dibucaine and three fluorescent probes (i.e., berberine, palmatine, and coptisine) for occupancy of the cucurbit[7]uril (CB[7]) cavity was studied by fluorescence spectra, UV-visible absorption spectra,1H NMR spectra, and theoretical calculations in acidic aqueous solution. Based on the fluorescence enhancement of berberine, palmatine, and coptisine upon binding with CB[7], respectively, a series of fluorescence detection methods for dibucaine were proposed. At the optimized conditions, the fluorescence intensity of berberine-CB[7], palmatine-CB[7], and coptisine-CB[7] complexes showed negative correlation to the concentration of dibucaine, which led to a series of simple and sensitive fluorescence methods for the determination of dibucaine for the first time. The linear Linear ranges obtained in the detection of the dibucaine were0.018-3.34μ mol L-1,0.032-4.47μ mol L-1, and0.079-4.42μ mol L-1with detection limits of6.0nmol L1,12.0nmol L-1, and25.0nmol L-1, respectively. Moreover, the proposed method was successfully applied for the determination of the drug in biological fluids. The competitive mode based on CB[7] superstructure provided a promising assay strategy for fluorescence detection in various potential applications. intetaction, and molecular catalysis within and between guests. The uses of CB[n] in these field are summarized. 3. Studied using fluorescence spectrophotometry weak fluorescence drug hydrochloride meclofenoxate for Cucurbit[7]urea-berberine clathrate fluorescence quenching. Changes in the value△F within the concentration range of0.028-2.90μmol L-1meclofenoxate of hydrochloric acid concentration and fluorescence emission signal exhibits a good linear relationship, the lowest detection limit of0.013μmol L-1. This approach has been successfully applied to the body in urine drug molecules trace analysis, recovery of96.0-102.8%.4. Fluorescence probe and nuclear magnetic resonance spectroscopy to study the hoist interaction between Cucurbit[7]urea and anti-TB drugs, isoniazid, and in the isoniazid gradually added to the cucurbit[7]urea-berberine fluorescent probe when the system, can be observed the fluorescence intensity of the fluorescent probe system sharply. Isoniazid competing drug molecules into the Cucurbit [7] urea body cavity to form a new stoichiometric ratio of1:1supramolecular inclusion complex, its inclusion stability constant K=(1.89±0.2)×1055M-1. For this competition system, established a quantitative analysis of aqueous solutions weakly fluorescent substance antituberculosis drug isoniazid high sensitivity and selectivity of the new fluorescent spectrophotometry. There is a good linear relationship between changes in the value△F concentration0.046-0.9μmol L-1concentrations within the range of isoniazid and its corresponding fluorescence emission signal, the minimum detection limit of0.015μmol L-1. This method has been successfully applied in pharmaceutical formulations and human urine isoniazid trace analysis, the accuracy and precision is good. In addition to the theoretical calculation and NMR cucurbit [7] the mechanism of urea and isoniazid was confirmed.