Studies On Security Of Small Molecular Bovine Acidic Neuropeptide

Biopeptides refer to the peptides that have beneficial or physiological effect on organism. In recent years, the research on biopeptides made rapid progress. It has been shown that biopeptides have the function such as immunomodulation, endocrine modulation, enzyme inhibition, antibiotic, antiviral, anticancer and flavour adjusting. Collectively, Biopeptides can be widely used in medicine and food industryA large number of chemicals can cause genetic lesion to some degree in human body, which lead to gene mutation and chromosome aberration. Long time touching mutagen can cause diseases such as cancer, teratogenicity and genetic deficiency. Some scientists suggest some antimutagens shoud be discovered to avoid the attack of mutagen on human body. Meanwhile, the research on mechanism of antimutation is one of the hottest fields.Neuropeptides, one group of biopeptides, are some important substances that have functions of neuronal signal transmission and regulation. They distribute widely in the central neural system, participating in a variety kinds of functional regulation. An Yuhui isolated some small neuropeptides from bovine brains and found a new peptide: bovine acidic neuropeptide 1 (BANP-1), a small molecular peptide without antigenicity. Experiments in animal model have proved that ﹊t can regulate neural system by increase amino acid and affecting the contents in cGMP in brain, (2) By increasing the synthesis of protein and the antioxidants defences of brain andimproving the energy metabolise of brain and decreasing the synthesis of No in brain, BANP-1 have good treatment on model of AD (Alzheimer's disease) rats and VD (Vascular dementia) mice.In this study, we measured the acute toxicity of BANP-1 and evaluate mutation and antimutation with micronucleus test of polychromatic erythrocytes on mouse bone marrow, Ames test and chromosome aberration test of Chinese hamster ovary cell. Meanwhile, we examined the activity of enzyme which have antoxidation and phase II biotransformation and observed the change with different drug adding way in Ames test to explain the mechanism of antimutation of BANP-1.Method: The acute toxicity of BANP-1 was investigated through measurement of LDso and MTD, and then black avoidance test was done to measure the change of remember, study ability that can reflect the role of BANP-1 to neural system.To understand antimutatiotf and mutation of BANP-1 in organism level, micronucleus polychromatic erythrocyte test was done to observe in mouse bone marrow. And, we examined the activity of antoxidation enzymes which includeing SOD, MDA, GSH-Px, FAOC, CAT and enzymes involved in phase II biotransformation including GST and GSH. The aim is to understand the mechanism of antimutation of BANP-1.To understand antimutation and mutation of BANP-1 in prokaryotic cell level, we detected the mutation and antimutation of BANP-1 with Salmonella typhimurium strain mutagenicity test (Ames test). In order to know the mechanism of antimutation of BANP-1, four different adding drug ways were used: A incubate mutagen and BANP-1 together for 30min, then add bacteria, B incubate BANP-1 and bacteria together for 30min, then add mutagen; C incubate mutagen and bacteria together for 30min, then add BANP-1, D incubate BANP-1, mutagen and bacterial together for 30min.In eukaryotic cell level, chromosome aberration test of Chinese hamster ovary cell was done to detect the mutation and antimutation of BANP-1.Result: 1 LD50 of BANP-1 cannot be detected and the MTD of BANP-1 is larger than 24g/kg. Comparing with normal group, BANP-1 groups had no significantdifferences (P>0.05) in black avoidance test.2. Micronucleus test: In mutation test, BANP-1 groups have significant differences (PO.05) comparing with positive group, and have no significant differences comparing with negative group. In antimutation test, BANP-1 groups have significant differences (PO.05) comparing with positive group. BANP-1 groups have significant differences (PO.05) in the activity of enzyme about antoxidation and phase...