| Objective Make a blunt trauma model in rat heart ; study pathological changes in normal and injured zone; to deduce the rule about MMP-1(matrix metalloproteinases-1) and MMP-2(matrix metalloproteinases-2) expression intensity ;try to find objective morphological foundation for blunt heart trauma in forensic identification and inquire possible pathogenesis.Methods 50 health and mature rats were classified into 7 groups with no purpose and were sacrificed 0h,6h 12h 24h,3d ,5d,7d .0h group is control group ,the others are experimental groups . Control groups have not been struck; which is different from experimental groups. Make specimens until experiment needed time; observe them with microscope and stain them with H-E method .we study the expression of MMP-1 and MMP-2 in heart with immunohistochemistry , then measure them by the computer image analysis and statistic analysis.Results (1) With time pass, the expressions of MMP-1 and MMP-2 show regular changes. The expressions of MMP-1 occur apparently 12h later after injured and escalate gradually, finally reach the extreme 3 days later. While the expressions of MMP-2 occur apparently 24h later after injured and reach the extreme 5 days later.(2)We can observe congestion, hemorrhage, infiltration with inflammatory cells and fibrosis in myocardium tissues with H-E method .(3)Compare statistic analysis between injured groups and control groups ,the result is p＜0.05.Conclusion (1) The injure model of heart is quite complete ;(2)There are relative concordances between H-E stain and immunohistochemitry in control and experimental groups. The distribution rules in each groups clarify that MMP-1 and MMP-2 can become detective marker to study myocardium injure.
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