| Purpose:Collectively, martial arts are ancient forms of Chinese traditional sports, where healthy weight and body fat levels are the basic requirement for athletes when completing difficult actions. Exploring the nutrition and metabolism of athletes is a way to improve the effectiveness of projects on an international level, for example, by conducting comprehensive dietary investigations amongst female martial arts athletes. To achieve the goal of this project, intensified (i.e. the process of adding a chain of amino acids) wheat germ powder has been added to the diet of martial arts athletes during training sessions, with subsequent observation of the effects on biochemical indexes and immunity function. This approach was expected to provide important theoretical support and experimental basis for promoting the practical application of immunological theory, strengthening scientific training and reasonable nutrition supplementation in martial arts.Methods:As research subjects for this project, twelve (12) well-trained female martial arts athletes from Shanghai University of Sports participated on a voluntary basis. They were exposed to a weighing method combined with food model and 24-hour review methods for investigation and study on meal diet, in addition to tests for detection of blood biochemical indexes-namely creatine kinase (CK), creatine kinase heart-shaped isozyme (CK-MB), blood urea and serum (by automatic biochemistry analyzer)-and immune indexes such as IgG, IgA, CD3, CD4, CD8, CD48, and NK determination (by flow cytometer). Furthermore, a comparison between two experimental groups was made (placebo vs. fortifier supplement) for effects on indexes such as CK, IgG, HB and PA.Results:1,The nutrition survey results show that (a) the female martial arts athletes' actual energy intake were significantly below recommend standard (28.17±12.63, 58.3±16.48 Kcal/kg/d, P<0.05); (b) carbohydrate, fat and protein intake were significantly lower than the actual recommended intake (CHO:3.96±1.8,6.98±1.27; PRO:1.0±0.38,1.68±0.3; Fat:0.94±0.48,1.55±0.28 g/kg body mass, P< 0.01); (c) the carbohydrate-fat-protein proportion of energy was 54:17:30, which is lower than recommended; (d) breakfast, lunch and dinner proportion of energy was 30%, 38%and 26%, respectively;(e) vitamins (Va, Vb1, Vb2, Vc, Vpp) and minerals (K, Na, Zn, Fe, Ga, Mg) intake indexes were significantly lower than recommended (P< 0.05)-only vitamin E and selenium consumption measured as recommended.2,Analysis of all the indicated blood biochemical indexes (using independent T-tests) shows that significant differences were found between index data before the experiment and after the recovery.3,Multivariate analysis of variance between the placebo (n=6) and fortifier (n=6) groups revealed the following:(a) The fortifier group had significantly higher levels than the placebo group (P< 0.05) in terms of the measured indexes on HB, IgG and PP [HB (141.08±9.20,127.58±6.90 g/L); PP (359.92±56.96,328.00±48.61 mg/L), IgG (8.42±0.81,7.20±0.73 g/L)]; (b) The fortifier group had significantly lower levels than the placebo group in terms of CK measurements (82.00±35.01,119.167±66.49U/L, P< 0.05). Conclusions:This project shows that the aggrandized wheat germ protein power supplementation helps to improve (1) protein levels (2) hemoglobin and (3) albumin levels of female martial arts athletes. In addition, the supplement has an activating effect on the immune function.
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