Effects Of Acute Bouts Of Swimming Exercise On CPT-1 Expression In Mouse Muscle

carnitine is the only transport for long-chain fatty acid in the mitochondria membrane,which plays an important role in the process of energy metabolism.The energy metabolism of long-chain acid was a limited step of long-chain fatty acid oxidation.CPT-I was alimiting enzyme for catalyzing the transport of long-chain fatty acid in the mitochondria membrane CPT-I could,however,be a molecule that has important regulatory roles in adjusting lipid oxidation during exercise,but there has seldom reports about CPT-ImRNA expressionand and protein content in response to variations in exercise duration.The purpose of this study was to determine the effects of the exercise (swimming) time on theCPT-I gene and protein expression in mice skeletal muscle,and the relationship between the expression of CPT-I and the plasma free fatty acid,plasma Leptin and insulin.The methodsof this study:CPT-Iexpression was measured after the same intensity swimming exercise at different times in mice quadriceps muscles of the C57 mice.90-minute swimming and exhausted swimming activated CPT-I in quadriceps muscles.The control group had none exercise.,the expression of CPT-ImRNAand proteinthe were measured,theplasma free fatty acid,plasma Leptin and insulin were also measured.The result of this study:1.90-minute and the exhausted swimming exercise resulted in a greater increase of muscleCPT-I expression compared with the control group,and the CPT-I expression was higher after exhaustive swimming than the 90-minute swimming.2 the plasma free fatty acid was elevated during exhaustive swimming,and there was a native correlation between the expression of CPT-I and plasma free fatty acid 3 the plasma Leptin was decented first,then elevated during exhaustive swimming 90-minute and the exhausted swimming exercise resulted in a greater decent of plasma Leptin compared with the control group,the plasma Leptin was higher after exhaustive swimming than the 90-minute swimmingThe conclusion of this study:the swimming exercise resulted in a greater increase of muscleCPT-I expression compared with the control group,The results suggested that the swimming exercise had effects on the skeletal muscle CPT-I expression;the plasma free fatty acid was elevated during exhaustive swimming;and the plasma Leptin was decented first,then elevated duringlong time exercise...