The Study Of Liqi Tiaobu Tang On Anti-fatigue Induced By Exercise And Its Mechanism In Rats

Objective:For the current research focus on Exercise-induced fatigue recovery, Through search information at home and abroad of Chinese medicine for anti-exercise-induced fatigue and NEI, Initial understanding of the relationship between them. This experiment in rat model of exercise-induced fatigue, Observe the effects of Liqi Tiaobu Tang decoction on NEI of the rat of fatigue induced by exercise. Reveal the mechanism of the decoction in the field of delaying fatigue, speeding recovery and improving physical function.Methods:30 male SD rats were randomly divided into 3 groups:control group 8, model group 11, medicine group 11. Medicine group ig the certain dosage Liqi Tiaobu Tang every day. Control group and model group ig the certain dosage Saline every day. In addition to control group, the rest rats trained for 6 weeks with swimming training method. By the end of the training, load 5% of their body weight. All rats were killed on 24 hours after last training, Taking blood 5mL from the abdominal aorta. Separate serum and Test the content ofβ-EP,C,T,LH and IL-2. Then separate hypothalamus, Chopped and Homogenate, centrifugal and get the supernatant. Using ELISA test the content of hypothalamusβ-EP.Results:(1) The time of the medicine group rats swimming to exhaustion was significantly prolonged compared with model group(P<0.01).(2) Compared with the control group, levels of serum T and IL-2 in the model group were markedly decreased(P<0.01), levels of serum C were markedly increased(P<0.01), T/C were markedly decreased(P<0.01). Rats fatigue caused by overtraining.Compared with the model group, content of serum T and IL-2 in the medicine group were significantly enhanced(P<0.05). Content of serum C were markedly decreased(P<0.01), T/C were markedly increased (P<0.01).(3) Compared with the control group and the medicine group, content of serumβ-EP in the model group were markedly increased(P<0.05). The content of hypothalamusβ-EP in the model group were markedly increased compared to the control group (P<0.05). Compared with the model group, content of hypothalamusβ-EP in the medicine group were slightly decreased, but the change isn't significant.Conclusion:(1) Liqi Tiaobu Tang can prolong the exhaustive swimming time. It can regulate the secretion of serum T,C,β-EP and hypothalamusβ-EP. Keep balance of the hypothalamic-pituitary-glandular axis.(2) Liqi Tiaobu Tang can enhance the activity of IL-2, play a role in regulating immune function. (3) Though regulating the function of nerve-endocrine-immune network, Liqi Tiaobu Tang can maintain system stability. It can prevent the exercise fatigue.