Effect Of Qingfei Tongluo Recipe On MAPK Signaling Pathway In Lung Tissue Of Young Rats With RSV Infection

Purpose:This study establish young Wistar rats model of respiratory syncytial virus acute lung injury by nasal inhalation.The expression of p P38、p ERK、p JNK in pulmonary tissue were assayed by immunohistochemical staining and western blot.Observe Qingfei Tongluo Fuxiong ointment efficacy to the model of respiratory syncytial virus acute lung injury and dose-effect relationship.Provide a reliable experiment basis for clinic about RSV related to acute lung injuryMaterial and method: 1. Apply the method of nasal inhalation respiratory syncytial virus long strains establishment the model of yong Wistar rat RSV infection.Observe daily the general state of young rats,such as diet,water,activity,fur,respiration, weight.lung general observation and light microscopy to identify the model. 2.Observe their lives condiction,survival amount every day.Combine the lung appearance and lung pathological section to investigate the therapeutic effects of the high dose group of Qingfei Tongluo Fuxiong ointment,the middle dose group of Qingfei Tongluo Fuxiong ointment and the low dose group of Qingfei Tongluo Fuxiong ointment. 3.Using the method of immunohistochemical staining and western blot to examine the leves of The expression of p P38、p ERK、p JNK in pulmonary tissue.Observe the therapeutic effects and dose-effect relationship of Qingfei Tongluo Fuxiong ointment to RSV infected young rats.Results: 1. The normal contorl subjects lives in good condiction,such as activities,diet and defecation regularly. Macroscopic lung tissue color is white, surface luster and moist.HE staining alveolar structure complete, consistent uniform size, no inflammatory cell infiltration.The model losts its shine,reduces its diet and water,feels under the weather,lags in response,loses weight. Macroscopic lung tissue color is crimson,edema.HE staining alveolar wall epithelial cell damage, severe alveolar wall thickening, interstitial lung visible inflammatory cellinfiltration. 2. Immunohistochemistry showed: Compared with the normal group,the levels of p ERK and p JNK in the other groups were significantly increased(P <0.05), the levels of p P38 in the model group and the low dose group were significantly increased(P <0.05); Compared with the model group, the levels of p ERK, p P38, p JNK in other groups were significantly different(P <0.05); compared with the high dose group, the levels of p ERK, p P38 and p JNK in the low dose group were significant difference(P <0.05); compared with the middle dose group, the levels of p ERK and p JNK in low dose group were significant differences(P <0.05), whereas no significant difference in p P38(P > 0.05). 3. Western blot results showed that: compared with the normal group, the middle dose group p JNK protein levels were not significantly different(P> 0.05), other groups p ERK, p P38 and p JNK protein levels were significant differences(P <0.05); compared with the model group, other each group decreased p ERK, p P38 and p JNK protein levels(P <0.05); compared with the high dose group, he middle dose group p ERK protein level was significant differences(P <0.05), compared with the high dose group, the levels of p ERK, p P38 and p JNK in the low dose group were significant difference(P <0.05); Compared with the middle dose group, the low dose group p ERK and p JNK protein levels were significantly differences(P <0.05),while p P38 no significant difference(P protein level> 0.05).Conclusion: 1.Using the mefthod of nasal inhalation could succeed in establishing the model of Wistar rats respiratory syncytial virus acute lung injury. 2. MAPK signaling pathways involved in the process of respiratory syncytial virus infection. 3.Qingfei Tongluo Fuxiong ointment is the treatment of respiratory syncytial virus infection effective for external use.Qingfei Tongluo Fuxiong ointment could in varying degrees reduce p ERK, p P38 p JNK levels in the lung tissue of young rats infected with RSV and there is a dose-effect relationship.