A New Design Of Software For Patch-Clamp System

The Patch Clamp Technique has become an essential method for cell electrophysiology research, which is widely used in the filed of the ionic channel current and the cell secretory mechanism. The patch clamp experiment software PClamp4.0, which is based on UDA-1 data acquisition, can meet the researchers'basic requirements in patch clamp experiments, but the structure of this software is too complicated, and high memory consumed when it is in operation. Therefore, it is not fit for long-time experiments and convenient for update and migration. For solving all these problems, a new edition of the software was designed in this research.In this thesis, the target system was analysed and class models were created by using Unified Model Language and Objected-Oriented Methodology. In the design, Reusable Object-Oriented modularization, including seal test mode, episodic mode, gap-free mode, hardware connection mode and so on, was adopted. Modularization design, which facilitates to read and test , can reduce the complexity of the program effectively. The software is a MFC application based on Windows system, and has the following features: less memory consumed, high speed, easy to update and migrate as a result of adopting thread synchronization with event kernel objects and share memory technology. Meanwhile, the design of convenient interface between operators and the computer offers a pleasant and easy operation environment.The experiment results have shown that the new edition software can output the stimulation waves exactly and record response of cell in real time. Compared with the original software PClamp4.0, the new edition software can meet the need of electrophysiology experiments in a more satisfactory way.