Effects Of Removing The Gut Symbiotic Bacterie On The Development Of Musca Domestica And The Parasitism Of Nasonia Vitripennis

Musca domestica is one of the most important sanitary pests in the world, and Nasonia vitripennis is an important natural enermy insect which can parasite the pupae of M. domestica. Main results were as follows:1. Effects of removing the gut commensal bacteria on the growth and development of M. domesticaThe developmental duration of M. domestica larvae increased significantly with the increase of ciprofloxacin concentration, and the durations of egg and pupae had no significant change. The adult pre-oviposition duration was prolonged, adult duration was shortened, and the fecundity was declined, but the fly could complete generation development. The age-specific survival rate (lx), the intrinsic rate of increase(r), the finite increase rate (λ), and the net reprodution rate (R0) were significantly decreased.2. Effects of removing the gut symbiotic bacteria on the biochemical substances in M. domesticaThe content and chemical property of M. domestica removing the gut symbiotic bacteria was determined. The trehalose content of larvae increased significantly in the first day after removing the gut symbiotic bacteria. After the gut symbiotic bacteria were removed, the trehalose content of pupa was not changed remarkably, the protein content of larva decreased significantly and CAT, POD, SOD activity increased significantly. The kinds and contents of amino acids in blood of M. domestica without gut symbiotic bacteria were not remarkable.3. Effects of proteomics of N.vitripennis on removing gut symbiotic bacteria of M domestica pupaWith the increase of concentration of ciprofloxacin removing of gut symbiotic bacteria in M. domestica, the parasitic rate and emergence rate of N.vitripennis decreased, female aduts duration were shorter than the control group, and the N. vitripennis development duration in M. domestica pupa significantly prolonged.4Effects of quantitative proteomics of M. domestica pupa on the of parasitism N. vitripennisResults showed that effects of the kinds and contents of amino acids in pupa on the parasitism of N. vitripennis were not remarkable. Protemic anaiysis showed that295kinds of protein expressed significant difference, and37kinds of protein expression quantity changed more than2times, most of the molecular weight were concentrated between41kDa and99kDa; Serine protease residues and thiocyanate protein expressed dropped and transmembrane protease serine, serpin serine protease inhibitor, ATP synthase, heat shock proteinxpressed increased.