The Establish And Study Of Cell Suspension Culture Mode In Microfluidic Chip

With the development and success of space technology and flight,our country has entered the stage of space researches and explorations.Since the complexity of space environment,the instruments for space loading experiments are restricted for some special conditions.However,the biological experimental methods on ground are lack of proper space loading instruments which means that they cannot play a role in space.The researches on explorations of small volume,low power consumption and automated instruments are under developing with the support of many national key scientific projects,like 973,863,National Sci-Tech Support Plan,etc.Microfluidic chips with the functions of microscale size,integration and high throughput,breaking the restriction of traditional biology,are ideal methods for space loading experiments.The controllable fluid and versatile micro-design can help build similar models of space biology.Related researches have indicated that there might be changes of immune functions under radiations and microgravity.To build up immune cell culture modes in microfluidic chip can help us understand the mechanism of space biological effect.What's more,by creating the culture modes,they can play an important role in breaking biological research area,like drug delivery,cell analysis and cell-cell interaction,etc.The research content mainly includes the following aspects:A microfluidic chip were proposed which can entrap cells and avoid fluid stress efficiently.Immune cell can maintain long term culture in the chip with perfuson.After the exploration of material and cell line,there had the first adopt to study the proper chip design.And the outcome indicated one of the key elements was effective holding-back design.Subsequently,PDMS chip provides the decisive influence of the flow rate which can damage the cell by shear stress.Finally,polyvinyl chloride fabricated microfluidic chip combined with bolting-silk was proposed and maintained long-term suspended cell culture.By finding a feasible medium component in CO₂ culture and in no-CO₂ culture,immune cell can maintain long term culture in polyvinyl chloride fabricated microfluidic chip conbined with bolting-silk with or without CO₂,which may give strong support for the study of cell culture in space environment.