Preliminary Research On Construction Of β-carbonyl-phthalocyanine Zinc And Carbon Based Nano-materials Complexes And Its Use As A Fluorescent Probe

There are strong UV-vis absorption in the600-800nm range, strongfluorescence emission and high fluorescence quantum yield for phthalocyaninecompounds, therefore phthalocyanine compounds are the research focus of thephotosensitizers in photodynamic therapy. However, its excellent optical propertiescan be used as markers of fluorescent probes. This article selects the single carboxylsubstituted phthalocyanine zinc (β-ZnPc) as the representative to preliminary studythe applications as fluorescent probes.In the first part, it preliminary study the UV-vis spectrum and established themethod for the determination of β-ZnPc molar absorptivity via UV-visspectrophotometric method.Through determination absorbance of the differentconcentrations β-ZnPc, the regression equation of absorbance–concentration was setup, the correlation coefficient r was0.9996, linear range was from1.6μmol/L to5.2μmol/L; the limit of detection was0.007μmol/L; the limit of quantification was0.02μmol/L; the day RSD and inter-day RSD were both less than5%; stability RSD notmore than1.44%. Method validation showed that the method was excellent specificityand accuracy and meets the quantitative requirements.In the second and third parts, we focused on the fluorescence changes ofβ-ZnPc as fluorescence probes before and after interaction with nano-materials toexplore the preparation conditions of the fluorescent probes. In the second part,materials are metallic carbon-based materials: homemade and commercializationnano-titanium carbide (TiC) and homemade nano-copper carbide (CuC). In the thirdpart, those nano-materials were replaced of non-metallic carbon-based materials:graphene and carbon nanotubes. The graphene materials contain three kinds of graphene. Then the carbon nanotubes include seven kinds of different lengths,diameters and walls carbon nanotubes among these carbon nanotubes, the2#carbonnanotubes divided into two: original and oxidized activation.In the second parts, it discussed the fluorescence changes of β-ZnPc asfluorescence probes before and after interaction with ametallic carbon-based materials.The experiments indicate that the fluorescence of β-ZnPc was quenched andfluorescence emission spectra red shift when adding varying amounts ofnano-materials. Under the same conditions, compared with the nano CuC andcommercialization nano-TiC, home-made TiC fluorescence quenching effect is moreobvious. The further study showed human serum albumin (HSA) and bovine serumalbumin (BSA) could desorption β-ZnPc from β-ZnPc-TiC system, make β-ZnPcfluorescence recovery.In the third parts, it juxtaposed the similarities and differences of the fluorescentprobes change. It found the fluorescence intensity of β-ZnPc significantly decreasedregardless of adding graphene or carbon nanotubes solution, and the latter wasstronger. The2#carbon nanotubes had the strongest fluorescence quenching effect inall materials. Therefore, the2#carbon nanotubes was activation by concentratedsulfuric acid, mixed acid (concentrated sulfuric acid: concentrated nitric acid=3:1). Itshowed that there was the better quenching effect for2#carbon nanotubes activationby concentrated sulfuric acid and the recovery was better after mixing with the HSA.