Study On Hypolipidemic Effects Of A Compound Of Seaweed Dietary Fiber

Hyperlipidemia refers to abnormally increased one or more levels of blood lipids,and it can lead to disorders, such as atherosclerosis, coronary heart disease, andpancreatitis. In this study, dietary fiber and traditional Chinese medicine which couldbe used for both medicine and edible were chosen to compose a compound ofseaweed dietary fiber (CSDF). And the lipid-lowering mechanism of the compoundwas discussed through experiments on hyperlipidemic rats in vivo and experiments onHepG2cells in vitro. The preventive and therapeutic effects were studied throughmulticomponent and multi-channel methods.According to the principles of traditional Chinese medicine prescriptionmedicine, CSDF was mainly composed of Gracilaria tenuistipitata agar, Gynostemma,Hawthorn and Cassia, and the proportion was4︰1︰2︰1. In this study, experimentsin vivo and experiments in vitro were combined to discuss the lipid-loweringmechanism of this compound through detecting serum lipids content, the degree ofliver damage, antioxidant activity and some other factors.Sprague-Dawley rats with hyperlipidemia induced by high-fat diet were used tostudy the hyperlipidemic effects of CSDF, all the rats were fed with different dose ofCSDF for8weeks. Results showed that total cholesterol (TC), triglycerides (TG),low-density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA), the activitiesof glutamic oxalacetic transaminase (AST) and glutamic-pyruvic transaminase (ALT)in serum were significantly decreased while high-density lipoprotein cholesterol(HDL-C), the activities of superoxide dismutase (SOD), glutathione peroxidase(GSH-Px), hepatic lipoprotein lipase (LPL) and hepatic lipase (HL) were significantlyincreased compared with model groups, and the results also showed a dose-dependentmanner. In addition, maximum dose was used to carry out the acute toxicity test. Theresults showed that CSDF had no toxicity.Hypercholesterolemia was simulated by the addition of cholesterol or serum forHepG2cell models. Experiments in vitro were conducted from lowering cholesterol and triglyceride. Results showed that the traditional Chinese medicine in thecompound of CSDF could reduce the concentrations of intracellular cholesterol andtriglyceride in a dose-dependent and time-dependent manner at the premise, whichthe dosage did not affect the proliferation of HepG2cells. Furthermore, thecompound could increase cellular antioxidant activities and reduce intracellularconcentration of acetyl coenzyme A (A-CoA). Among them, Gynostemma might bethe most pharmacologically active component of CSDF for decreasing theconcentrations of intracellular cholesterol, and Hawthorn might be the mostpharmacologically active component for lowering triglyceride.Experiments both in vitro and in vivo showed that CSDF could significantlyreduce serum lipid levels on hyperlipidemic rats, facilitate the repair of damaged cells,decrease membrane permeability, hinder the flow of cholesterol into cells andimprove cellular antioxidant activity. These findings indicated that CSDF had apositive therapeutic impact on mixed hyperlipidemia. The compound improved theadded value of Gracilaria tenuistipitata agar and provided a viable basis for theclinical treatment of hyperlipidemia.