Preparation And Photothermal Therapy Investigation On Prussian Blue Nanoparticles Modified By Hyaluronic Acid

Due to environmental pollution and fast pace of modern life, cancer hasbecome the most deadly disease in recent years, it kills about7.9million patientsthrough the world in2007, and13%of the total number of death. This number willincrease to12million in2030. Clinical treatment of the cancer at present is mainlythrough the methods such as surgery, chemotherapy and radiotherapy. However, dueto cancer’s metastasis and infection, cancer cannot be cured by simple surgerycompletely, meanwhile chemotherapy and radiotherapy also cannot cured cancerideally because of the poor specificity of tumor tissue in the terminal cancer. Inrecent years, a new cancer treatment of tumor (photothermal therapy, PTT) hascaused the attention of scholars, PTT is a new minimally invasive tumor treatment,which is by converting light energy into heat energy through the photoabsorbentagent.However, the emerging technology has some problems and deficiencies, suchas high cost of light conversion agent, complicated preparation process, poorspecificity and unapproved biological safety. Therefore, the development of novellight conversion agent has become the research focus in recent years.In this paper, we successfully synthesized prussian blue nanoparticles modifiedwith fluorescein isothiocyanate (FITC) and hyaluronic acid (HA)molecules(PB@PEI-FITC-HA NPs) through a simple boiling reflux method andcondensation reaction of amino and carboxyl.The nanoparticles are averagely50nmin diamiter, and its Zeta potential at different synthesis stages proves the successlymodification of FITC and HA molecules. Photothermal therapy experiments showedthat PB@PEI-FITC-HA NPs can convert light energy into heat energy effectively,killing HeLa cells under near infrared laser irradiation with high thermal stability.By contrast, PB@PEI-FITC-HA NPs had very low toxicity to HUVEC cell in theabsence of near-infrared light irradiation. Targeted photothermal therapyexperiments of different tumor cells showed that PB@PEI-FITC-HA NPs can bebetter uptaken by HeLa cells, with more obvious therapy ablation effects to HeLacells compared with U87MG cells, which suggested that PB@PEI-FITC-HA NPshave the ability to target and kill tumor cells by photothermal therapy.The results in this work showed that the PB@PEI-FITC-HA NPs were a kind oflight conversion agent which has cancer targeting ability. And the nanoparticles hadvery good prospect in clinical application because of its simple preparation process,low cost, stable chemical structure and high biological safety.