Preparation And Application On Photothermal Therapy Of Multifunctional Nanoparticles Based On Prussian Blue

Photothermal therapy has been widely studied as a new cancer treatment method for its unique advantages of safety and non-invasive.Now,various nanomaterials can be designed as photothermal transducing agents with specific optical,physicochemical,biological and pharmaceutical properties.However,there is still little research that could achieve the integration of efficient synergistic tumor therapy into a single nanoplatform.In this paper,two kinds of JNPs based on Prussian blue(PB)NPs were successfully synthesized.At the same time,their abilities of photothermal conversion,drug delivery and tumor ablation in vitro and in vivo were studied.The main research results are as follows:Firstly,we prepared unique Prussian blue@polyacrylic acid/Au aggregates Janus nanoparticles(PB@PAA/Au-A JNPs)by coating PAA preferentially on one of the flat surfaces and subsequently forming small Au NPs of 2-4 nm inside the PAA network.The obtained JNPs not only realize the optimum integration of PB NPs and Au-A,but also combine their multi-functionalities effectively to achieve diagnosis and treatment of tumor.Importantly,attributed to the extraordinary heterostructure,the exposed surfaces efficiently maintain the original photothermal properties of PB domain and Au domain and has the function of drug loading at the same time,which almost cannot be realized simultaneously in common core-shell structure.Besides,benefited from the Janus structure,the coupling effect of two distinct PB NPs and Au-A domains leads to a superior photothermal conversion efficiency(?)of 49.4%,which increases by 9.9% compared with pure PB NPs.Collectively,the synthesized doxorubicin-loaded PB@PAA/Au-A JNPs can be used as p H and NIR dual-stimuli responsive drug nanocarriers with the capacities of simultaneous computed tomography imaging,chemotherapy and enhanced photothermal therapy,which achieved excellent tumor inhibitory effect.Secondly,the Prussian blue@polyacrylic acid/Cu S JNPs(PB@PAA/Cu S JNPs)were successfully synthesized by a facile approach,in which PB had a photothermal conversion capability in the near-infrared first(NIR-I)window and Cu S had a photothermal conversion capability in the near-infrared second(NIR-II)window.Based on the synergistic photothermal effect of Cu S NPs and PB NPs,the as-prepared PB@PAA/Cu S JNPs exhibited strong absorption effects in the NIR-I and NIR-II double bio-windows.Besides,benefited from the Janus structure,the coupling effect of two distinct PB NPs and Cu S domains leads to a superior photothermal conversion efficiency(?)in the NIR-I and NIR-II double bio-windows,which increased by 4.6% in NIR-I bio-windows compared with pure PB NPs and increased by 3.9% in NIR-II bio-windows compared with pure Cu S NPs.Collectively,the synthesized PB@PAA/Cu S JNPs can be used as an excellent photothermal transducing agent in NIR-I and NIR-II double bio-windows for photothermal therapy,which achieved excellent tumor inhibitory effect.