Effects Of Dynamic High Pressure Microfluidization Combining With Glycation On Antigenicity, Functional Properties And Structural Changes Of β-lactoglobulin

Milk proteins allergy has attracted people all the time. β-lactoglobulin, which isthe major allergen, has high nutritional value and good functional properties. Sincethe antigenicity limits the application of β-lactoglobulin in the food industry, it’surgent to figure out effeetive way to make use of β-lactoglobulin. In this study,β-lactoglobulin was modified by dynamic high pressure microfluidization combiningwith glycation treatment, and the effect on antigenicity, functional properties andstructural changes of β-lactoglobulin were investigated.The results of study were as follows:(l) Effect of dynamic high pressure microfluidization combining with glycationtreatment on the antigenicity of β-lactoglobulin: β-lactoglobulin was modifiedgalacto-oligosaccharides, xylo-oligosaccharides,1-Kestose,1F-Fructofuranosylnystose, and galacto-oligosaccharides decreased β-lactoglobulin antigenicity the best.Then, β-lactoglobulin was treated by previous dynamic high pressuremicrofluidization treatment(0.1,40,80,120,160MPa) and subsequent glycation withgalacto-oligosaccharides(GOS). The antigenicity of β-LG-GOS decreased at relativelow pressures (≤120MPa), especially at80MPa. The rise of molecular weight wasdetected by polyacrylamide gelelectrophoresis to ensure glycation.(2) Effect of dynamic high pressure microfluidization combining with glycationtreatment on the functional properties of β-lactoglobulin: after combining treatment,the solubility, emulsion stability and antioxidation of β-LG were improved. Solubilityimproved best at80MPa. However, the emulsion capacity decreased.(3) Effect of dynamic high pressure microfluidization combining with glycationtreatment on the structural changes of β-lactoglobulin: after combining treatment,β-LG sulfhydryl group content increased and surface hydrophobicity decreased. But itvaried according to pressure levels. Additionally, fluorescence spectra, UV absorption,and circular dichroism (CD) analysis indicated important tertiary and secondarystructural changes of β-LG.