Synthesis And Performance Of Molecularly Imprinted Polymers Of BPA

Molecular imprinted as a novel, highly efficiency recognition technology, had beattract the world’s attention from scientific research institutions and developingrapidly for that molecular imprinted polymers (MIPs) possess high selectivity andsensitivity for target molecular. Molecular imprinted technology have been widelyused in medical and technological field such as: chromatography, adsorbents toenvironmental estrogens, filtration or adsorption bimolecular, etc.Molecularly imprinted polymer (MIP) was synthesized throughphotopolymerization by using BPA as templates,2-VP as functional monomer, AIBNas initiator and EGDMA as crosslinker. Typically，using n-hexane and toluene asporogen. The different proportions of the porogen and different polymerizationtemperatures on the preparation of molecularly imprinted polymer (MIP) wasinvestigated. The morphology of MIP was analysed through scanning electronmicroscopy (SEM) and was preliminary studied the imprinting properties of MIP viaUV-Vis spectrophotometer. The results of SEM indicated that MIP using n-hexaneand toluene as porogens with a proportion of4:1did not need grinding and sievingand particle size was relatively uniform. The Scathcard analysis showed that there aretwo kinds of affinity locus sites. The dissociation constant of high affinity’s bindingsite Kd1was476μmol/L and the max apparent binding capacity Qmax1was184.5mg/g(0.808mmol/g); while the dissociation constant of low affinity’s binding site Kd2was568.9μmol/L and the max apparent binding capacity Qmax2was257.532mg/g(1.128mmol/g).When the analogue of BPA is used as competitive substrate, thepolymer has good specific adsorptive ability on template and thus can be adopted inthe separation of analogue of BPA.Molecularly imprinted polymer (MIP) was synthesized with BPA，MAA，AIBNand EGDMA as templates, functional monomer, initiator and crosslinker, respectively.Furthermore, the morphology of polymeric microsphere is represented through theadoption of SEM. The adsorptive capacity of polymerization system on BPA and itssubstrate is studied through static adsorption experiment and selective experiment ofsubstrate. Scatchard analysis is carried out afterwards. The Scathcard analysis showedthat there are two kinds of affinity locus sites. The dissociation constant of high affinity’s binding site Kd1was436.7μmol/L and the max apparent binding capacityQmax1was109.0mg/g (1.27mmol/g); while the dissociation constant of low affinity’sbinding site Kd2was1.32mmol/L and the max apparent binding capacity Qmax2was146.0mg/g (1.7mmol/g).The results indicated that the polymer has good Specificselectivity adsorption ability on BPA.There were prepared a novel monomer (N-methyl-l-phenylalanine) tosynthesized imprinted molecularly core–shell microspheres on silica gel. In addition,the recognition selective ability, adsorption capacity, Scatchard analysis and kineticswere investigated. The dissociation constant of high affinity’s binding site Kd1was12.39μmol/L and the max apparent binding capacity Qmax1was11.52mg/g; while thedissociation constant of low affinity’s binding site Kd2was200.0umol/L and the maxapparent binding capacity Qmax2was350.8mg/g.The results show that imprintedpolymer has good adsorptive capacity and specific recognition ability on template. Agreat synergistic effect and electrostatic interaction due to the novel monomer workedin adsorption capacity.