Separation Of River Heptachlor Degrading Bacteria And Its Degradation Influence Factor Research

In this study, the natural anaerobic microorganisms taken from WeiHe River sediment iscultured and acclimated, then isolated and purified. Three strain which has the ability ofdegradation is isolated from the microorganisms. After that, we degrade heptachlor in thesame conditions, measure and compare the rate of degradation. And then screen out degradingbacteria that can be able to degrade heptachlor efficiently. Besides that, study the effects ofdifferent factors on the microbial degradation of heptachlor, obtain optimum environmentalconditions of predominant strains for degrading heptachlor, providing some referencemethods and theoretical basis for microbial degradation of heptachlor.In this study，we can draw the following conclusions:1. The bacteria source of the experiment is from WeiHe natural Microorganism. Culturemicroorganism in sealed dark conditions at the temperature of15℃and pH value of7. In thedomestication process, through continuous improvement heptachlor’s concentration of culturemedium, screened out microbial flora which can degrade heptachlor in an anaerobicenvironment.Degrading bacteria final acclimated concentration is0.2mg/L;2. Isolating and purifying the microbial flora of domestication, get three different strains(Z1, Z2, Z3). Degrade for ten days at a temperature of15℃, pH value of7, heptachlor’sinitial concentration of0.2mg/L and anaerobic conditions. Degradation of strains Z2is best,is the screened predominant strains of the experiment;3. The experimental study on the effect of different temperatures, different pH, differentexternal carbon source, different initial concentrations of heptachlor, different amount ofmicroorganism and Degradation of heptachlor mixing different strains of dominant strains.Obtaining optimum conditions of strains Z2for the degradation of heptachlor: temperature of25℃, pH value of8.0, adding glucose as external carbon source, heptachlor’s initialconcentration of0.2mg/L, stock solution of dominant bacteria volume of100mL, thedegradation of single strain Z2.These results indicate that degrading bacteria of heptachlor can be filtered out fromWeiHe mature sediment. Screened degrading bacteria Z2has a good effect on the degradation of heptachlor.