Separation Of Heptachlor Degrading Bacteria And Its Degradation Characteristics Research

OCPs is a natural or synthetic organic pollutants, has persistent for a long time, half highvolatile, bioaccumulation and toxicity, can long distance migration through the environmentmedium and its serious hazard to human health and the environment. Seven chloride is one ofthe OCPs, it to human beings and their living environment has serious destructive, difficult todegradation in the environment, biodegradation of OCPs research at home and abroad isrelatively broad, but not seen research on biodegradation of heptachlor.In WeiHe river polluted seriously mature microorganisms as a source of bacteria in thesediment, heptachlor to typical persistent organic pollutants, adopt the method of anaerobicdegradation, for natural microorganism domesticated, four heptachlor strains were isolatedfrom mature sediment degradation bacteria, degradation experiment under the anaerobicenvironment, to get advantage degradation bacteria. Respectively in different temperature,different pH, heptachlor initial concentration, of advantage strain degradation effect. Study ofheptachlor advantage bacteria optimum growth conditions, and in optimal conditions inability, and the advantage species are16s rDNA identification.The study of microbial degradation of heptachlor and prevention and governance ofheptachlor engineering research laid a solid foundation, to control these substances emissionsand biological treatment technology provides an important basis, to the environment ofheptachlor biodegradation has the positive significance.This experiment study eventually come to the conclusion as follows:1. Screening strains of advantage: this experiment from the wei river sediment in fourkinds of single strains were isolated from mature number (H1, H2, H3, H4), respectively tofour kinds of single strain degradation experiment, under the condition of the pH value of7.0,the temperature is20℃, and seven chlorine concentration is0.1mg/L, the anaerobicdegradation after10days, H1, H2, H3, H4strains of degradation rate were46.09%,52.7%,56.0%,38.24%, obviously H3bacteria degradation rate is highest, as the experimentaladvantage;2. Strains H3bacteria species identification: advantage after16s rDNA appraisal, and the database of known bacteria homology is very low, belongs to new strains of unknown;3. H3bacteria optimum growth conditions, it is respectively in different temperature,different pH, degradation experiment under different initial concentration of heptachlor,heptachlor advantage bacteria optimum growth conditions for pH value:8.0, temperature:30℃, heptachlor, initial concentration of0.3mg/L;4. H3bacteria optimum growth conditions in effect: in the pH value:8.0, temperature:30℃, heptachlor: initial concentration of0.3mg/L, under the condition of degradation after10days, heptachlor degradation rate as high as80.01%;5. H3bacteria mixed with the other three kinds of bacteria:under the condition of thepH value of8.0, the temperature of30℃, heptachlor initial concentration of0.3mg/L, willbe heptachlor advantage bacterium and purification of other three kinds of bacteria,anaerobic degradation after10days, found not blend of heptachlor degradation rate is lowerthan the dominant strains of heptachlor maximum degradation rate was80.01%, visible thereis competition between other bacteria strains and advantage, the advantage bacteriumdegrading heptachlor have inhibition.Mature from the wei river sediment in the screening of strains of heptachlor advantage isfeasible, under anaerobic conditions, from the wei river of mature sediment filter have beenthe advantages（H3） of heptachlor has good degradation effect.