PFOS And PFOA On Genes Expression Of Stress Related Protein And DNA Damage Of Planarian Dugesia Japonica

Perfluorooctane sulfonates(PFOS) and Perfluorooctanoic Acid(PFOA) whichwere the representative of Perfluorinated chemicals(PFcs) had been widely used inautomotive paints,textile,non-stick coating and other products because of theirhydrophobization and dustproof since1950s’.As the toxicity of PFcs was decovered,PFOS and PFOA have been disabled,but their toxic mechanisms are not clear.Dugesiajaponica is an original three mesoderm animal with the strong regeneration ability.And it can be used in detecting water quality for sensitive to environmental changes.In this study,planarians were exposed to PFOS and PFOA solution,the relationsbetween the expression of tress related genes and the drug concentration or the timewas expored.The DNA damage was detected when planarians were exposed to PFOSand PFOAin different long time.Complete planarians and regenerating planarians were exposed to six dilutePFOS and PFOA respectively.Then changes of tress related genes expression weredetected with RT-PCR, the genes functionations were cell stress resistance,neuraldevelopment,apoptosis,signal transduction.Results showed that planarians exposed inlower concentration of PFOS and PFOA for short time,the expressions of hsp70andhsp90mRNA decreased,and increased as higher concentration and longer time thatexpression was maybe activated by oxidative stress.The numbers of p53mRNAexpressions were increased caused by higher concentration PFOS and PFOA for10-days.That indicated planarians begin to apotosis for two drugs stress.The twodrugs had little effect on Xnp related nerve.And the expression of wnt signal wasinduced by PFOAand not PFOS.Complete planarians and regenerating planarians were exposed to five dilutePFOS and PFOA respectively.The numbers of HSP70protein expression weredetected with Western Blot.The results showed there was not correlation betweenHSP70protein expression and the concectration of PFOS and PFOA or time longer incomplete planarians.But the expression of HSP70was induced by PFOS and PFOA inregenerating planarians.This result was not consistent with trend in hsp70mRNA,sowe speculated that the two drugs could effect on process of translation,and the numberof HSP70increased to protect from toxicity of PFOS as that was accumulated inplanarians,and related with regeneration. The nuclear DNA damage was detected when planarians were exposed to PFOSand PFOA solution for1-day,4-days,7-days and10-days.To explore the toxic effectsof the two drugs on nuclear DNA.The results showed that PFOA could make DNAdamaged in about20%cells of all tested,but PFOS could not.In order to confirm ourrosults,the depurated coli DNA was incubated with PFOS and PFOA solution.Itshowed that PFOAmaked DNAbroken and PFOS didn’t.