The Preliminary Study Of Biological Effect Of Static Magnetic Field On Hydra Magnipapillata And Dugesia Japonica

The effect of static magnetic field intensity on survival, population growth, asexual reproduction and activities of two kinds of antioxidant enzymes of Hydra magnipapillata and Dugesia japonica was observed by the method of population accumulative culture. In addition, the effect of static magnetic field and magnetized water on head regeneration process of Hydra magnipapillata and Dugesia japonica were also investigated. The results showed that:1. The static magnetic field had a significant influence on the asexual budding reproduction and the activity of two kinds of antioxidant enzymes of Hydra magnipapillata. After 23 days, the hydra population density in all the magnetic exposure gradients was higher than the control gradient. From the 3th day to the end of the experiment, the hydra population density was always the highest at the static magnetic field of 190mT. In addition, the instantaneous population growth rate of hydra in the control gradient was lower than that in the magnetic exposure gradient. The hydra instantaneous population growth rate of the control group and the static magnetic field of 190mT were the biggest in the 7th day, however that decreased after 7 days; the Hydra instantaneous population growth rate of the static magnetic field of 310mT and 450mT were the biggest in the 9th day, however that decreased after 9 days; while the instantaneous population growth rate of the static magnetic field of 418mT was the biggest in the 11th day. Moreover, with the increasing magnetic field intensity, the activities of SOD and CAT had the decreasing trend.2. The static magnetic field had obvious effect on asexual reproduction by transverse fission and the activity of two kinds of antioxidant enzymes of Dugesia japonica. After 15 days, the Dugesia japonica population density in all the magnetic exposure gradients was higher than the control gradient, and with the increasing of magnetic field intensity, the activities of SOD and CAT had a decreasing trend. The results showed that: The static magnetic field of 200mT-500mT could promote planarian asexual reproduction by transverse fission, but inhibit remarkably the activities of two kinds of antioxidant enzymes. Low dose of magnetic treatment might have a stimulating effect on the asexual reproduction of planarian.3. The static magnetic field and magnetized water had obvious effect on hydra head regeneration. In the condition of static magnetic field, the magnetic treatment promoted head regeneration process at 8h-24h; after 28h, the rate of Hydra regeneration in all the experiment groups was lower than the control group. In the condition of magnetized water, the magnetized water could promote hydra head regeneration process at the early regeneration stages, but inhibit the rate of head regeneration at late regeneration stages.4. The static magnetic field and magnetized water had a significant influence on the head regeneration of planarian. There were some differences in planarian head regeneration process under different intensity of magnetic treatment. In the whole of head regeneration development process, the rate of head regeneration at the static magnetic field of 400mT-450mT was faster than the other experiment groups, and the effect was significant. The magnetized water could also promote planarian head regeneration process. With the increasing of magnetic field intensity by which culture water was magnetized, the effect of promotion was strengthened significantly. Moderate magnetic treatment (static magnetic field and magnetized water) could promote head regeneration process and accelerated wound healing.