Distribution Characteristics Of Mineral Elements In The Growth Period Of Hops

Hops are one of the main raw material of beer brewing with variouspharmacological activities, and also an important economic crops in Xinjiang, Manyfactors will impact the growth and metabolism of hop plants, such as soil, water andmineral elements in the surrounding environment. The present study dealt with threemain hop varieties and several important growing stages of two different areas grownin Xinjiang：Analysis of qualitative and quantitative characteristics of mineralelement, distribution, migration and its correlation; investigation the mineralelements content between hop varieties and hop growing areas by using maincomponent analysis and cluster analysis; evaluation the relationships among themineral elements and others hop active components, including hop resins, hoppolyphenols and flavonoid. The main results of researches are as follow：1. A method based on wet digestion and inductively coupled plasma-massspectrometry (ICP-MS) was established for simultaneous determination of twenty-twomineral elements (Al, Pb, As, Cd, Ba, Ni, Mn, Cu, Sr, Co, Cr, Zn, Li, V, Fe, Ga, Rb,Cs, K, Ca, Na, Mg) in different parts of the hops. The linear correlative coefficient forabove elements were higher than0.999(R2), and the detection limits were range from0.12μg/L to4.08μg/L. The relative standard deviation was less than5%withrecovery rates of87.35%-110.11%. The method was accuracy and credible.2. It is found that the content of mineral elements is significantly differentbetween soils and plant’s fraction derived from different hop growing regions. Theidentified hops characteristic elements were confirmed by combining both principalcomponent analysis and cluster analysis, namely Al, Ni, Mn, Co, Fe, Ga, Cs, Ca, Mg,Sr, Li, Rb, Na, K. The result of cluster analysis can effectively distinguish the hopsregion of hops planting, which would expect a basis theoretical reference for thetraceability in the different sources of hops origin and could largely enrich thefingerprints of hop mineral elements. But it need further study to make a clear distinction between varieties of hops.3. The migration and distribution behaviors of the22kinds of mineral elementsin different plant were investigated in the hop plant growth cycle, which distinguished7stages: germinating stage, lead shelf period, cut bud, climbing period, bud burstingperiod, enlargement period and picking period. The regulation was obtained onabsorption, migration and distribution of above-mentioned elements in the hops. Itwould be provide a theory support for the actual production and scientific fertilizerplant.4. The correlation analysis were carried out by using the methods of correlationcoefficient and typical correlation analysis among the hop resins, total flavonoids,total polyphenols and22kinds of mineral elements content in three hop varietiesgrown in different areas. The results of correlation coefficient showed that there weresignificant correlations between18kinds of minerals and hop resins or total hoppolyphenols. Furthermore, typical correlation analysis between minerals elements andhop active components indicated that:(1) The weak correlation was found between total flavonoid and mineralelement;(2) Ba and Li has positive relationship with the α-acid, and V, Rb, Mg shows thenegative relationship on the α-acid;(3) Ba, Sr, Mg has positive relationship with the β-acid and total polyphenols,and Li shows the negative relationship on the β-acid and total polyphenols.