Preparation And Characterization Of Self-assembly Based Multicolor Fluorescent β-cyclodextrin Polymers

As the rapid development of life science and technology and the rapid increase ofanalysis objects, it put forward higher requirements on the detection and analysistechnology and is in urgent need of analysis methods with short time, low cost andhigh sensitivity. Multicolor luminescent materials with multi-distinguishable emissionsignals under a single wavelength excitation can be used to simultaneously analyzemultiplex targets or trace multiple biological processes, so they can effectively reducethe time, the sample volume and the cost of analysis. Multicolor luminescentmaterials have great application value in the field of analysis and detection.In recent years, the technology of preparing multicolor luminescent materials hasmade great progress. Existing multicolor luminescent materials are mainly preparedbased on the size effect or optical coding technology, but these methods have someshortcomings. The types of multicolor luminescent materials based on the size effectare not enough, and part of them have poor biocompatibility; coding methods basedphysically entrapping may lead to localized collection of fluorescent substances; themulticolor particles are unstable based on electrostatic adsorption, hydrophobicinteractions or hydrogen; encoding method based on covalent crosslinking orpolymerization requires a relatively complicated procedure. So, in this paper, wedeveloped an optical encoding method to prepare stable multicolor-cyclodextrinpolymer (poly-β-CD) based on self-assembly. The details are summarized as follows:1. A new method for preparing multicolor fluorescent poly-β-CD based onself-assembly has been developed. Based on the principle of three-primary colors(RGB), naphthalene, adamantane-labeled fluorescence (ADA-F) and pyrene butyricacid were selected as three-primary colors (red, green, and blue, respectively). Andpoly-β-CD was used as carrier for dyes. Dyes can self-assemble on poly-β-CD basedon host-guest interaction between the cavity of-CD and dye. Seven different colors(magenta, blue, light blue, white, green, yellow and red) of encoded poly-β-CD can beobtained by changing the molar ratio of the three dyes. In the principle ofthree-primary colors, more colors of poly-β-CD can be obtained by controlling theratios of the three-primary colors. This multicolor fluorescent poly-β-CD based onself-assembly has many advantages, such as facility, easy control, high photostabilityand so on, and it will be used as multicolor material for multiplex labeling in high-throughput screening and imaging fields.2. A method has been developed for preparing pH-sensitive and multicolorfluorescent poly-β-CD based on self-assembly. Using the host-guest interaction of thecavity of-CD and adamantane, pH-sensitive green ADA-F and pH-insensitive redadamantane-labeled fluorescence (ADA-R), ADA-F and ADA-R have stablyself-assembled on poly-β-CD. Multicolor fluorescent poly-β-CD with highphotostability can be obtained by changing the ratios of ADA-F to ADA-R. When thedoping ratio of ADA-F to ADA-R is1:2, fluorescent poly-β-CD can respond to widerange of pH values from4to9. Meanwhile, the multicolor fluorescent polymer hasbeen successfully applied to code agarose gel microsphere, and it is expected to beused in multiplex analysis and detection of pH in complex system.