Fermentation Complete Piglet Feed Through Lactic Acid Bacteria And Yeast Isolated From Koumiss

Nine samples of lactic acid bacteria and seven of yeast isolated from koumiss were the strain samples for tested.The first experiment was that the yeast produce protease’s in qualitative and in quantitative experiments, then the lactic acid bacteria and the yeast were activated after the three generations of continuous culture, which was the inoculums were added to the compound feed sterilized of10%,the mixed compound feed was fermented respectively in37℃and28℃for3days, the number of living bacterium, the pH value, the lactic acid and the recovery rate of dry matter as an index on feed fermentation, selected the excellent fermentation feed strains. The results showed that the comprehensive index fermented fine feed strains,they were lactic acid bacteria strains1-3,5-1-1and yeast strains J2,the J20.The pH values of lactic acid bacterial-3,5-1-1on fermented feed were4.95±0.07and5.02±0.03; the count of the living-yeast J2, J20were1.2×109CFU/g and1.1×109CFU/g.Secondly good strains should were domesticated, separately with the solid, the semi-solid and liquid domestication method. Experimental results showed:the liquid domestication method for four strains of domestication, its effect was obvious. After the domestication, the pH value of lactic acid bacteria1-3fermentation feed was4.89±0.05,the pH value of lactic acid bacteria5-1-1fermentation feed was4.99±0.06;the count of the living-yeast J2of fermentation feed was1.3×109CFU/g, the count of the living-yeast J20of fermentation feed wasl.4×109CFU/g. Liquid domesticated method can be widely applied.Finally for the screening complex strains of excellent strains screened from single strain screening experiment combinatorial, They were determined for each combination of the total number of viable cells, the lactic acid bacteria viable count, pH, the lactic acid degree, the dry matter recovery, the in vitro dry matter digestibility and the in vitro digestibility of crude protein, select a combination of good bacteria. The results showed that:the excellent strain combination was1-3+J2, the total viable count was6.05×108CFU/g, the lactic acid bacteria single viable count was3.30×108CFU/g, the pH value was5.21±0.01, the lactic acid degree was1.01%±0.09, the dry matter recovery was94.58%±0.01, the in vitro dry matter digestibility was67.12%±0.25, the in vitro digestibility of crude protein was80.42%±0.14.