The Research Of Ozone Degradation Mycotoxin And Other Detoxification Methods

Influenced by growing environment, processing and storage, the mycotoxin in food, suchas aflatoxin B1(AFB1) and zearalenone (ZEN), was very high. A large nμ mber ofmycotoxin left in food caused serious damage to animal and hμ man health. How todegradate mycotoxins has attracted broad attention. This paper mainly studied the effect ofozone (O3), chlorine dioxide (ClO2) and microwave on the degradation rate and the optimaldegradation conditions of AFB1and ZEN, analysed their degradation products and assessedtheir toxicity based on the mouse model and Ames model. The main research resuts asfollows:(1) This paper explored the degradation rate and the optimal degradation conditions ofAFB1in peanut meal degradated by O3, analysed the degradation products, and evaluated theirsafety. The optimal condition was12min processing time,15%water, the ratio peanut mealamount to container volume5:500g·mL-1. Under this condition, the degradation rate of AFB1was85.01%. Three degradation products were discovered through LC-MS in AFB1afterdegradation by O3. The results of mice experimenttal show that there was no significantdifference compared the blood indicators and the pathological of liver, kidney, thymus andspleen of mice fed peanut meal degradated by O3.with the blank group of mice, they did nothave the symptoms of AFB1. The results of Ames experimenttal show that the products ofAFB1degradated by O3.stimulused the strains defect histidine, strains didn’t mutations andform colonies, degradation products of AFB1didn’t have mutagenicity.(2) This paper explored the degradation rate and the optimal degradation conditions ofZEN in corn starch degradated by O3, analysed the degradation products, and evaluated theirsafety. The optimal condition was45min processing time, the ratio corn starch volμ me toadding water volμ me was20:30mL·mL-1, the ratio corn starch volume to container volume17:500mL·mL-1. Under this condition the degradation rate of ZEN was92.41%. Threedegradation products were discovered through LC-MS in ZEN after degradation by O3. Theresults of mice experimenttal show that there was no significant difference compared theblood indicators and the pathological of liver, kidney, thymus and spleen of mice fed peanutmeal degradated by O3with the blank group of mice, they did not have the symptoms of ZEN.The results of Ames experimenttal show that the products of ZEN degradated by O3stimuuledthe strains defect histidine, strains didn’t mutations and form colonies, degradation products ofZEN didn’t have mutagenicity.(3) This paper explored the degradation rate and the optimal degradation conditions ofZEN in corn starch degradated by ClO2, analysed the degradation products, and evaluatedtheir safety. The optimal condition was150min processing time,160μg·mL-1chlorine dioxideconcentration,30℃, Under this condition, the degradation rate of ZEN was97.01%. Twodegradation products were discovered through LC-MS in ZEN after degradation by ClO2. Theresults of mice experimenttal show that there was no significant difference compared theblood indicators and the pathological of liver, kidney, thymus and spleen of mice fed peanut meal degradated by ClO2with the blank group of mice, they did not have the symptoms ofZEN. The results of Ames experimenttal show that the products of ZEN degradated by ClO2stimulused the strains defect histidine, strains didn’t mutations and form colonies, degradationproducts of ZEN didn’t have mutagenicity.(4) This paper explored the degradation rate and the optimal degradation conditions ofZEN in corn starch degradated by microwave, analysed the degradation products, andevaluated their safety. The optimal condition was180s processing time,550w microwave,the ratio corn starch volume to water volume was10:5mL·mL-1. Under this condition, thedegradation rate of ZEN was85.05%. Five degradation products were discovered throughLC-MS in ZEN after degradation by microwave. The results of mice experimenttal show thatthere was no significant difference compared the blood indicators and the pathological of liver,kidney, thymus and spleen of mice fed peanut meal degradated by microwave with the blankgroup of mice, they did not have the symptoms of ZEN. The resuls of Ames experimenttalshow that the products of ZEN degradated by microwave stimulused the strains defecthistidine, strains didn’t mutations and form colonies, degradation products of ZEN didn’t havemutagenicity.