Effects Of Cyclocarya Paliurus (Batal.) Iljinskaja Polysaccharide On Lipid Metabolism And Related Genetic Expressions In Rats

Polysaccharide is one of the important active ingredients of Cyclocarya paliurusleaves. This study was conducted to investigate the antihyperlipidemic and antioxidanteffect of Cyclocarya paliurus (Batal.) Iljinskaja polysaccharide(CPP) in high-fatemulsion-induced hyperlipidaemic rats. We examined the effects of (CPP) on weight gain,serum levels of the total cholesterol (TC), triglyceride (TG), low density lipoprotein(LDL-C), high density lipoprotein (HDL-C) and blood antioxidant enzymes. Theexpressions of HMG-CoA, FAS, ATGL, PPARα, Glut-4were investigated by Q-PCR toexplore the effect of CPP in liver and white adipose tissue of hyperlipidemic rats. Theprotein expression of related genes were performed by western-blotting. The resultsshowed as follows:(1) During the animal experiment, Cyclocarya paliurus (Batal.) Iljinskajapolysaccharide showed significant hypolipemic effect. Serum TC and TG levelswere significantly lowered by CPP compared to the model group(P<0.01) and inmost cases were indistinguishable from rats fed normal chow, basal diet(normalcontrol group). Conversely, levels of high density lipoprotein-cholesterol (HDL-C)were elevated in the groups given daily doses of CPP(P<0.01), compared to thehyperlipidemic model group.(2) Activities of total superoxide dismutase (T-SOD), glutathione peroxidase(GSH-Px) and catalase (CAT) in serum were significantly elevated in CPP-treatedgroups while levels of malondiadehyde (a byproduct of lipid peroxidation)decreased(P<0.01or P<0.05).(3) CPP could significantly inhibit the body weight gain in rats with hyperlipidemia(P<0.01) and significantly reduce the aliphatic index (P <0.05).(4) Cyclocarya paliurus (Batal.) Iljinskaja polysaccharide could significantlyenhance the mRNA expression of ATGL, Glut-4, PPARα(P<0.01or P<0.05) andrestrain the mRNA expression of FAS (P<0.01) and HMG-CoA in liver and (or) inwhite adipose tissue of hyperlipidemic rats.(5) CPP could significantly enhance the protein expression of ATGL, Glut-4, PPARαand restrain the protein expression of FAS, HMG-CoA in liver of hyperlipidemicrats (P<0.01or P<0.05). Cyclocarya paliurus (Batal.) Iljinskaja polysaccharide could also affect the protein expression of FAS, ATGL, HMG-CoA, PPARα,Glut-4in other organs of hyperlipidemic rats.