Magnification Of Biodiesel Synthesis Process Using Immobilized Lipase

This paper studied the synthesis of biodiesel from waste oil in organic solvent system using immobilized lipase from Candida sp.99-125. The effect of various factors on reaction was studied, including the inhibition of short chain alcohols on enzymatic reaction, substrate concentration, water content, adding methanol and so on. A optimum condition was as the following:Using petroleum ether as the organic solvent, the molar ratio of waste oil and methanol is1:3;adding methanol in separate three times; adding10%～15%(percent of weight of waste oil) water; the amountof substrate is1:5～1:6to waste oil(v/v); the reaction temperature is40℃.In the scale up of biodiesel synthesis process using immobilized lipase, we can draw the conclusions as follow:With raw soybean oil being the feedstock, the performance life of the immobilized lipase reached more than5batches.The total amout of immobilized lipase is56kg(the activity of lipase is8000IU/g), and the amount of raw product we collected can reach to870litres.And the average convertion can reach to80%. With swill oil provided by Shanghai Luming environment science company being the feedstock, the performance life of the immobilized lipase reached more than6batches.The total amout of immobilized lipase is58kg(the activity of lipase is8000IU/g), and the amount of raw product we collected can reach to1030litres. A optimum condition was using petroleum ether as the organic solvent, the reaction temperature is35℃; the molar ratio of substrate and waste oil is1:4(v/v);water is added into the reaction in one time,and the amount is10%to the weight of the waste oil;the molar ratio of methanol mole equivalent of the raw oil;Adding methanol in separate three times the methanol and adding water in one time.But this method would consume more substrate,so in the following experiments with acidiferous oil provided by Qinhuangdao Leading Science and Technology Development Company, we used the molar ratio of substrate and waste oil is1:1(v/v), the other conditions of reaction were not changed.And a new feeding way, that is feeding from low inlet, was used in the following reaction. The performance life of the immobilized lipase reached more than5batches.The conversion of biodiesel was below80%after2batches,and it was below40%after5batches. In order to increase the blending effect of oil phase and water phase,a new solvent will be used in the next reaction.Using biodiesel as the organic solvent, the molar ratio of substrate and waste oil is1:1(v/v); adding10%wt (percent of waste oil) water; adding methanol in separate three times; the molar ratio of waste oil and methanol is1:3; the amount of lipase is40%to the weight of waster oil;the reaction temperature is40℃. As can be seen from the results, the conversion rate reached60%under the optimum conditions.In the scale up under this systerm,we can confirm the best reaction condition:the temperature had a little changed to35℃,the other conditions had same of data from lab. The activity of immobilized lipase is13000IU/g.Tthe conversion rate can maintain60%under the optimum conditions during384hours reaction...