Research And Improvement Of Evaluation Methods On Anti-fungalactivity Of Textile

The antibacterial and anti-mould textiles are important parts of functional textiles, so theactivity evaluation methods are particularly prominent for the developing and applying. The currentevaluation methods on antibacterial are relatively fast, simple and easy to operate, however, theevaluation methods on antibacterial activity have complicated steps, long experimental period, andcan not be quantitatively evaluated, therefore the original method need to be improved or a newevaluation method should be introduced.In this paper, Aspergillus niger, Penicillium chrysogenum and Candida albicans were chosen asthe testing strains, the reason of the low increment of Candida albicans control sample and the variedoperating conditions of GB plate method for anti-mold activity evaluation were investigated, and thetwo systems were optimized. ATP luminescence applied in antifungal evaluation were established.The GB plate method and ATP luminescence method were compared to evaluate the anti-fungalactivity after being finished by SCJ-2001.By researching the preparation methods of Candida albicans suspension, oscillation temperature,oscillation time, sugar content inside the dilution, it turned out the most important reason thatleading to the low increment of Candida albicans control sample was there’s not enough nutrition forCandida albicans to grow. Therefore,100%SDB culture medium should be prepared to dilute theCandida albicans suspension.The Aspergillus niger and Penicillium chrysogenum were chosen as testing strains to researcheffects of the inoculation methods, the volume of spore suspension and whether there’s glucose inthe culture medium.It turned out: whether there’s glucose in the culture medium had no significanteffects on the test results for both strains, therefore the culture medium should be withoutglucose.The inoculation methods only effected the Penicillium chrysogenum. Since in actual tests, the incubated spores suspension were mixed strains, the suspension should be incubated on thesurface of the testing sample. Though volume of spore suspension had no significant effects on thetest results, for samples with high water absorption, the volume should be adjusted to prevent theuneven spread of the srtain spores.The optimism extraction conditions of intracellular ATP inside Aspergillus niger, Penicilliumchrysogenum and Candida albicans were explored, and established the ATP luminescencemethod.After incubating0.2mL spore suspension with appropriate concentration into sample vialswith0.20±0.02g sterilized cotton fabric inside, placed them into25±2℃incubator, for Aspergillusniger and Penicillium chrysogenum,the incubating time was2d, and for Candida albicans was24h.Then the boiling, phosphate buffer, trichloroacetic acid, dimethyl sulfoxide and benzalkoniumbromide (BAC) five extraction methods were adopted to explore the optimum extraction conditionsof the intracellular ATP inside these three strains inoculated on cotton fabric sample. It turned outBAC is the best extraction method for three testing strains, and the best extraction condition was0.30%~0.50%BAC, pH12~13.5,3~5min extraction.Compared agar plate method and ATP luminescence method evaluating the antifungal activityof the cotton fabrics finished by SCJ-2001anti-fungal finishing agent.The optimum concentrationfor Aspergillus niger and Penicillium chrysogenum is7g/L and8g/L by agar plate method,thetesting time was28d.And the optimum concentration for the three mixed strain suspension was5g/Lby ATP luminescence method,the testing time was2d.So ATP luminescence method was not onlyfast but also visible sensitive than agar plate method.