| Use of cellulose decomposing strains biological compost is agricultural solid waste resource utilization is an important way. Cassava lees is the hotspot of bulk agricultural products processing by-products, manufacturing organic fertilizer is the main way of utilization, but there is no special efficient fermentation strains. Taking cassava residue as carbon source, with domestic and international more coverage on Aspergillus niger, Trichoderma konigii and Phanerochaete chrysosporium and other8similar strains of cellulose decomposing strain as the objects, through the single strain and composite strains by solid state fermentation of cellulase activity, cellulose degradation rate and crude fiber degradation rate and other indicators of decomposition effect evaluation, set of three superior composite flora, two single strain and a blank,so there are six treatment in total used in cassava dregs compost:in physics、chemistry and biology index evaluation of compost maturity. The main conclusions are summarized as follows:(1) Based on the study of single cellulose degradation strain, the results showed that the11strains of agent in cassava residue by solid fermentation process has better enzyme production capacity and cellulose degradation ability. The highest CMC enzyme activity and paper enzyme activity were respectively6.93U/ml and3.09U/ml by Phanerochaete chrysosporium and Trichoderma konigii at fifth day. At tenth day, weight loss rate, crude fiber and cellulose decomposition rate of cassava dregs were higher than the fifth day category, that the disparity were9.26-11.81%,16.92-16.75%,16.98-25.08%, respectively.(2) Structure the11strains of single strain according to orthogonal table flora construction, results showed that the amount of microbial and enzyme activity of composite strains were higher than single strain. The highest CMC enzyme activity and paper enzyme activity of composite strains were4.7times and8.3times higher than corresponding maximum enzyme activity of single strain. In composite strains, the highest crude fiber degradation rate was42.89%by flora12, the highest cellulose degradation rate was46.11%by flora4, they were higher than the single strain by2.16%and0.21%. Identified three superior flora respectively:Phanerochaete chrysosporium+Sporotrichum thermophile+Aspergillus niger+Saccharomyces cerevisiae+Streptomyces ambofaciens; Trichoderma konigii+Aspergillus niger+Saccharomyces cerevisiae+Bacillus subtilis+Streptomyces ambofaciens; Sporotrichum thermophile+Aspergillus ficuum+Aspergillus oryzae+Saccharomyces cerevisiae+Bacillus subtilis.(3) The evaluation of application effect of cellulose degradation composite strains showed that the pile temperature, pH and water content had same change trend, but amount of microbial were improved and the pile composting were speeded up by strains, and shorten the cycle of composting1-2d. Among the treatment, treatment2(Trichoderma konigii+Aspergillus niger+Saccharomyces cerevisiae+Bacillus subtilis+Streptomyces ambofaciens) application effect was the best and CMC enzyme activity was2.92U/ml, cellulose degradation rate reached20.88%, hunmic acid content was10.08%, higher than other treatment by0.16-0.52U/ml、2.35-9.21%、0.3-2.16%, respectively. |
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