Preparation Of Biological Composite Nanofibers By Electrostatic Spinning And Its Performance

Tissue Engineering (Tissue Engineering) is the application of cell Engineering and biology to research the substitutes of biological activity using repairing or improving the structure and function of damage to tissues or organs The basic principle and method is that the vitro amplification of the normal tissue cells are adsorbed on the biological materials which have good cell compatibility and can be absorbed by the body degradation, then the composite is implanted in the parts of the damage organization. Cells are proliferated gradually on the biological materials to form a new organization which has the morphology and function in accordance with corresponding tissues and organs when the biological materials are absorbed by organism degradation, so as to achieve the aim of repairing trauma and restore function.Electrostatic spinning is refers to the polymer solution or melt spinning process under the action of applied electric field. Under the effect of electric field force, the polymer solution or melt droplets in spinning nozzle overcome own surface tension and form the charged trickle which split times during the process of jet trickle. The non woven superfine fiber membrane are collected on the receiving screen by the solvent evaporation or curing. Due to the fibres obtained by electrostatic spinning are tenuous than conventional preparation method, so have the superhigh specific surface area and porosity. Electrostatic spinning is wide application in biomedical materials.In this paper, the nanometer fiber membrane of PLA/silk fibroin biomaterials, polycarbonate/gelatin were prepared by the method of electrostatic spinning and its properties were studied, the specific content is as follows: (1) Silk fibroin and polylactic acid were dissolved respectively using trifluoroacetic acid (TFA) and hexafluoroisopropanol (HFIP) and the orderly nanometer fiber membrane with different ratio, the solution concentration, spinning voltage and distance were prepared by the electrostatic spinning method. The fibers morphology were investigated.(2) It was proved through the biological experiment (toxicity test, etc.) that the silk fibroin/poly lactic acid fiber membrane are non-toxic and compatibility, so they belong to the safe implant materials. In the same cultivate conditions, a contrast with disordered fiber membrane culture were made.(3) Aloe with diminish inflammation was bringed into spinning solution and the polycarbonate/gelatin/aloe vera gel fiber membrane were prepared. The influence of different ratio fiber membrane on morphology, mechanical property and degradation rate were investigated. The oral mucosal cell culture on the polycarbonate/gelatin/aloe gel composite fiber membrane were done.