Study On Preparation And Performance Of PLA/Silk Fibroin Electrospinning Fiber Membrane

The fiber membrane prepared by electrospinning has a porous structure and is favored by researchers in the medical field because of its large specific surface area.In this paper,poly(lactic acid)/silk fibroin(PLA/SF)electrospun fiber membrane was prepared by electrospinning technology,The composition,surface morphology,crystallinity,hydrophilic properties,porosity,internal structure,drug release performance and antibacterial performance of the fiber membrane were studied,mainly including the following aspects:Firstly,the PLA/SF blended electrospun fiber membrane was prepared.The water absorption becomes stronger with the increase of SF in the fiber membrane.and increased polymer concentration leads to increased fiber diameter.When the concentration is 7.4%,the receiving distance is 15 cm,and the voltage is 10-20 k V,a radial beaded structure appears on the surface of the fiber membrane,and the water absorption and porosity are increased compared with the fiber membrane without the beaded structure.The PLA/SF fiber membrane after heat treatment has increased crystallinity and decreased porosity and water absorption compared with before treatment.After methanol solution treatment,the crystallinity of the fiber membrane with PLA/SF=7/3 increased.Secondly,the PLA/SF blend drug-loaded electrospun fiber membrane was prepared using voltaren as drug model.The surface morphology changes of the fiber membrane and the effect on the in vitro drug release performance were investigated by changing the PLA/SF ratio,concentration and spinning voltage.The fiber membranes prepared under all conditions are smooth fibers,and the more SF content in the fiber membrane,the faster the drug release rate.Changing the concentration,spinning voltage,and other factors will slightly change the drug release rate of the fiber membrane.The cumulative drug release rate of the blended fiber membranes under all preparation conditions reached more than 90% in 600 min after adding SF,and the release was sufficient.After heat treatment and methanol treatment,the drug release rate of the PLA/SF fiber membrane was reduced,and the initial release was effectively controlled.Finally,the PLA/SF coaxial drug-loaded fiber membrane was prepared,and the surface morphology,internal structure and in vitro drug release properties of the fiber were investigated by changing the ratio of PLA/SF in the shell or core layer.The PLA/SF coaxial drug-loaded fiber membrane has an obvious core-shell structure,and the drug is coated on the core layer,and the cumulative drug release rate is reduced compared with the blend fiber.The cumulative drug release rate reached 50%-70% at600 min,which was 30%-40% lower than that of the blended drug-loaded fiber,the problem of large initial drug release of the blended fiber was improved.When the components of the core layer and the shell layer are exchanged,the drug concentration gradient between the core layer-the shell layer,and the shell layer-the sustained release medium will also change accordingly.The change of the drug concentration gradient will cause the drug to be between the media.The release rate of the drug changes to affect the drug release behavior.As the SF content of the core layer increases,the drug release rate of the fiber membrane in the first 300 minutes tends to be faster than that before the core-shell components are exchanged.Using PLA loaded with nano-ZnO as the shell layer and PLA/SF=3/7 as the core layer,the electrospun coaxial drug-loading fiber membrane was prepared.It has obvious antibacterial property effects on Staphylococcus aureus and Escherichia coli,the best antibacterial property is when the content of nano-ZnO is 4%.