Preparation And Properties Of Polylactic Acid/silk Fibroin Composite Fiber Membrane By Electrospinning

Blending the two materials can overcome and improve the poor performance of single-phase materials,give full play to the advantages of each phase,and obtain composite materials that can meet different purposes.As a new type of material composite technology,electrospun has gained wide interest from researchers due to its ease of operation and wide adaptability for preparation on the nanometer scale.It can be applied in fields such as biomedicine,environmental protection,energy and food packaging materials.In this paper,polylactic acid(PLA)from a wide range of sources was electrospun and compounded with natural silk fibroin(MSF)and wild silk fibroin(TSF)to prepare polylactic acid/bombyx mori fibroin and PLA/TSF composite nano/micron fiber membranes.Based on modern analytical methods,the surface morphology,structure,phase state and other microstructures and interaction mechanisms of PLA/silk fibroin(SF)composite fiber membranes with different ratios were studied.At the same time,the thermal stability and decomposition mechanism,hydrophilicity and in vitro degradability and cell compatibility were experimentally analyzed,and they were practiced with the microstructure of the material.In addition,the mechanism function and action mechanism of the decomposition reaction are also analyzed.The main results are as follows:1.The electrospun method successfully prepared the PLA/SF composite fiber membrane.The study found that when the composite fiber membrane is mainly composed of polylactic acid,the surface of its single fiber has a unique pore structure.As the content of silk fibroin increases,the stability of the material increases.At the same time,there are crystalline phases,mobile amorphous phases and rigid amorphous phases in the structure.With the increase of the silk fibroin content in the composite film,the glass transition temperature(T_g)of the composite material shifts to a high temperature,the specific heat capacity increases,the crystallinity decreases,and the amorphous state increases.2.There is no obvious hole structure in the PLA/TSF composite fiber membrane,and the fiber surface is swollen and smooth.At the same time,under the same mass ratio,the?-sheet content in the ethanol-treated and untreated polylactic acid/wild silk fibroin is greater than that of the PLA/MSF composite membrane;after washing with water,the solvent residue can be effectively removed Ca²⁺.3.Compared with the degradation in water,the solution added with proteinase K can promote the degradation of the composite membrane.The higher the SF content in the composite membrane,the stronger the hydrophilicity and the more obvious the degradation.The unique hole structure on the fiber surface increases the specific surface area of the material,which is conducive to cell adhesion and differentiation,and provides enough space for its passage.In the range of 450-650K during the heating process,the best kinetic model is G(?)=?,f(?)=1 and lnA=35.58.This thesis explored the preparation method of polylactic acid/silk fibroin composite fiber membrane,and studied the internal relationship between the change of macroscopic physical quantity and the micro-molecular structure of the material,in order to further prepare polymers,proteins and their composite materials with adjustable structure and performance.Laid a theoretical and experimental foundation.