Research The Antioxidant Activity Of Lignans Extracted From Different Sesame Oil

Sesame seeds are rich in active substances, sesame lignans occupy part of the vast.Different process of sesame oil not only differ in quality and flavor, but also differ incomposition of sesame lignans and activity. There are many physiological activity of lignans,this paper focuses on the effects of processing to the antioxidant of lignans, in order toprovide the theoretical basis for the development and utilization of lignans and improveutilization of the value of sesame. At present, water extraction process and hot pressingprocess are the domestic processing technology, cold pressing method is also fast rise, so thispaper chose these three processing methods preparing sesame oil, and ethanol extractionmethod to prepare lignans, and then experiments around their antioxidant.First, lignans were extracted from different sesame oil with ethanol, and analyzed withhigh performance liquid chromatography (HPLC). Find that the ethanol extraction was mainlysesamin and sesamolin, the sesamin and sesamolin contents of these three lignans sampleswere52.66%,22.13(from water extraction process sesame oil);52.87%,23.02%(from hotpressing process sesame oil);54.46,25.62%(from from cold pressing sesame oil).Compared the antioxidant of the different processes sesame oils and the sesame lignansextracted from them in vitro. Soybean and peanut oil were used as the base material oil anddifferent sesame oils were added in them. Detect the oxidative stability by Rancimat-743, andfind that the hot pressing sesame oil enhance maximum oxidation induction time, followed bywater extraction process sesame oil, cold pressing sesame oil worst. Lard oil was used as thebase material oil and oven experiments was used to detect the antioxidant of sesame lignanssamples, the results was all three samples showed weak antioxidant in lard, the lignansextracted from hot-pressing sesame oil slightly stronger.To investigate the extracted sesame lignans differences in free radical scavenging, DPPHradical, hydroxyl radical and superoxide anion scavenging experiments were designed. The results showed that, in DPPH radical scavenging experiments, lignans extracted from waterextraction process sesame oil showed best, IC50was0.80mg/mL; follwoed by the lignansextracted from hot pressing sesame oil, IC50was2.00mg/mL; lignans extracted from coldpressing sesame oil showed worst. Hydroxyl radical scavenging experiments wereconsistent with DPPH radical scavenging experiments, superoxide anion scavengingexperiments maneuverability and poor reproducibility, difficult to find out a regular pattern.D-galactose was used as the Experimental model, Kunming male mice was used an theexperimental animals, the three samples were given high (120mg kg-1d-1), medium (60mg kg-1d-1), low (30mg kg-1d-1) three doses by orally way to study the antioxidant activityin vivo. By analyzing the activity of GSH-PX in the blood of mice and MDA content, T-SODactivity and GSH-PX activity in liver, to study the three extracted lignans exhibitedantioxidant activity in mice, the results show three lignans extracted （sesamin basis for thesame dose）, lignans extracted from cold pressing sesame oil exhibited the best oxidationresistance, the lignans extracted from hot pressing sesame oil secondly, lignans extractedfrom water instead sesame oil exhibit the worst oxidation resistance. Concluded that waterextraction process and hot pressing method undermined part of the sesame lignans, reducedoxidation resistance in vivo, cold pressing method can better protect oxidation resistance invivo.......................