Study On Preparation Of Chitosan Microsphere Carrying Efes And Its Ability In Vitro

The chitosan microspheres were prepared by ultrasonic emulsification method and TPP method. As model drug, BSA was encapsulated and adsorbed into micropheres respectively. The microspheres were dipped in physiological saline, then the ability of carrying drugs and sustained releasing of the microspheres was evaluated by drug loaded rate, encapsulation efficiency and releasing rate. The optimized factors of ultrasonic emulsification method are O:W is 15:1,stir speed is 600r/m,adding glutaral 1mL,treated by ultrasonic for 5 min,the loaded rate and encapsulation efficiency of the microsphere at this condition were 50.6% and 48.7% respectively.The optimized factors of TPP method are the TPP concentration is 1mg/mL,stir speed is 600r/m,adding glutaral 0.7mL after the reaction, the loaded rate and encapsulation efficiency of the microsphere were 50.2% and 37.4% respectively.The chitosan-phospholipid complex and N-alkylated chitosan were prepared.The chitosan-phospholipid microsphere made by ultrasonic emulsification method pocessed the best load rate and encapsulation of BSA, they were 60.1% and 61.8% respectively. the microsphere made by the same method carrying EFEs achieved the higher loaded rate and encapsulation efficency,which was 68.1% and 62.8%, the release rate after 72h is below 65%. The enzyme activity of EFEs were tested before and after the preparation. The enzyme activity did not change a lot.The result showed that the factors of the microsphere preparation were moderate and the activity of EFEs were not destoried much.