Synechocystis 6803 For The Biological Template Preparation Of Silica Microspheres And Its Application In Protein Separation Applications

Now with the research development in field of biomedicine and bioengineering, people are exploring various functional silica microspheres. In this paper, Study on Preparation and Morphology of the silica microparticles and hollow silica microspheres were carried.The silica microparticles with chemical modification of with agarose can serve as protein purification and separate the mixed proteins of BSA and Lys. so,It can be used for the fast separation and purification of bioengineering products.Different morphologies of silica were prepared using biotemplate via a facile sol-gel route. The monodisperse silica microparticles and hollow silica microspheres were successfully prepared by Synechocystis 6803 as bio-template, tetraethyl orthosilicate (TEOS) as precursor, and combined with sol - gel method. The different morphologies of silica were dependent on experimental conditions and the types of catalysts.The morphology and structure of Synechocystis 6803 and as-prepared silica were studied by means of Scanning electron microscope (SEM), Transmission electron microscope (TEM), Fourier Transform Infrared Spectrometry (IR), thermogravimetry (TG), X-ray diffraction (XRD) and Field emission scanning electron microscopy (FESEM),size distributions and energy dispersive X-ray spectrometer(EDX).. Reaction conditions of selective synthesis of the monodisperse silica micropartilcles and the hollow silica were established. The formation mechanism of different morphologies was discussed. The results indicated that the silica microparticles had an amorphous spherical structure with a diameter range of 1.5μm, retained the original morphology of the cells. SiO2 nanoparticles could be uniformly coated on the surface of cells to form the hollow SiO2 microspheres, which had uniform wall thickness and could maintain the original morphology of cells. It was confirmed that formation mechanics of the SiO2 hollow spheres and SiO2 microparticles were based on the difference between adsorption and penetration.In the alkaline environment, Sepharose-6B crosslinked by 1,4-Butanediol diglycidyl ether were grafted withγ-aminobutyric acid (GABA). In water-in-oil emulsion, 6% agarose grafted with GABA was dispersed on the surface of silica to prepare the complex of SiO2/agarose. The results show that the dispersibility of silica improved. The morphology of the silica observed with FESEM is excellent global, the surface of silica is rough which were covered with a similar granular substance. It was made 10×20mm Chromatography column with 3.5g SiO2/agarose dry powder. Outflow liquid was gradually collected every ten minutes. The outflow solution was detected with spectrophotometer to measure the absorbance. The protein content of the fermented broth was estimated by Coomassie brilliant blue staining by colorimetry. The recoveries of BSA and Lys were 90.0% and 87.0%, respectively. The result shows that silica microparticles can be used as a kind of new stationary phase for the electrostatic ion chromatography to purify protein Mixture to achieve the aim of concentrating and purification of protein.