Of Ampelopsin Inclusion Compound And Its Antioxidant Activity

Flavonoids is a large number of the active ingredients of chinese herbal medicine, It has wide range of physiological activity, thus in recent years, universal attention by researcher. Ampleopsin belongs to a kind of flavonoids, which shows very high biological activities. It can be used as food preservative and antioxidant in food industry. Now the research is focusing on DMY is about pharmacological aspect. In order to make the best use of DMY, it is very necessary to investigate the stability of ampleopsin under different condition and application character of it. And then these research giving a theoretical basis to the development and utilization of ampleopsin.This thesis is constituted by four chapters:In chapter 1,firstly, the flavonoids of the structure and classification are described in detail. In addition, introduce the more comprehensive to the nature of ampelopsin, pharmacological activity and status of current research.In chapter 2, firstly the inclusion complexes compared with solution mixing method and grinding method in different ratios. It was evaluated by infrared spectroscopy (IR), powder X-ray diffraction (XRD) and environmental scanning electron microscope(SEM). In addition, through ultra-violet spectroscopy (UV) and phase-solubility study of the inclusion complexes were investigated to determine the inclusion stability constants. Experimental results show that the inclusion complexes prepared by grinding solution method is better than mixing method, all inclusion complexes followed AL type which suggested the presence of inclusion complexes of ampelopsin and cyclodextrins with molar ratio of 1:1.In chapter 3, six inclusion complexes of ampelopsin and HP-β-CD with suitable interval degrees of substitution were prepared by the grinding method. The complexes were characterized by infrared spectroscopy (IR), powder X-ray diffraction (XRD), ultra-violet spectroscopy (UV) and environmental scanning electron microscope(SEM). The phase-solubility profiles of ampelopsin with the six HP-(3-CDs were investigated to determine the inclusion types and calculate the apparent stability constants (Kc). The results showed that the inclusion complexes were produced. Phase-solubility studies revealed the ampelopsin of a 1:1 complex of the AL type. The most abundant by HP-β-CD with DS=6.8.In chapter 4, In acetic acid medium, the hydroxyl radical (·OH) produced by Fenton system could change the color intensity of Amaranth. The change in absorbance was measured atλ= 510nm, and the·OH produced could be determined indirectly. By measuring conditions were studied to determine the optimal experimental conditions system. Under the selected conditions, good relationship were obtained between the scavenging effects of the hydroxyl free radical and the antioxidation, the experimental determination scavenging function of the inclusion of several flavonoids inclusion complexes followed the flowing order:Ampelopsin> Hesperetin> Noroxylin>5-Methyl-7-methoxy-isoflavone> Ipriflavone>Formononetin.In chapter 5,to investigate the stability of ampleopsin in different pH value, temperature and different metal ions.The experimental result shows:Ampelopsin in the aqueous phase as time and temperature changes will become unstable. pH value is also influence the stability of ampeloosis, as an important factor of the pH value increases, snake meat will accelerate oxidation, the structural will be changed and will decompose completely when the pH-value was above 9.0 weakly acidic environment and neutral conditionthe ampelopsin shows good stability, so it application and storage of suitable in this conditions. Metal ions Na+,Mg2+,Zn2+and Ca2+within 6h to ampelopsin were not significantly influenced in the stability, but the metal ion namely Al3+and Cu2+had significant effection, it will promote the ampelopsin oxidative and should avoid contact in applications.