Garlic Reactive Protein Extraction, Separation And Preparation Process Of Research

The active component of garlic lyophilized powder which was obtained using membrane separation method, the excipient ratio in the preparation engineering, effects on the tablet dissolution rate of the coating material weight was studied in this research. And the formula of the lyophilized powder preparation and the engineering parameters were determined, and the quality assessment of the tablet was performed at last.Superoxide dismutase (SOD) and soluble peptide was separated and extracted from garlic using membrane separation method. Next, garlic lyophilized powder got from the garlic concentrated solution using lyopyilization method. The SOD activity was tested using the classic adjacent pyrogallol since oxidation method. And the kinds and the relative molecular weight of the active protein in the garlic lyophilized powder were tested with SDS-PAGE method.Mixing garlic lyophilized powder with different kinds of excipents, using Jade bamboo polysaccharide solution as coating material, garlic tablets were made with wet granulating method. In order to get better preparation parameters, central composite design method was used to design experiments. The preparation formulas and the engineering parameters were optimized combining with the Creating response-surface design method. According to the experiments which were designed above, the ratio of the Microcrystalline cellulose (MCC) and lactose, and the coating material weight was determined. Bradford method was used to test the protein content to determine the dissolution rate. Finally, the experimental results were put into the SAS software to build dimensional linear fit function, create response surface and contour maps to get the final optimization parameters of preparation and engineering.The study suggests that the excipent ratio and coating material weight has an obvious effect on the tablet dissolution rate. And compared to the coating material weight, the excipent ratio plays a more important role. The optimized ratio of MCC and lactose is 57:43. To validate the experimental result, the coating material weight increased by 10%, and final result suggests that the experimental values are very close to the predictive ones. And the tablet appearance, hardness, weight variances all conforms to the Chinese Pharmacopieas.In addition, the garlic tablet made with above methods can well mask the pungent smell and be better for administration.