Screening Of Cellulose Degradation Baterrium And Its Application In Composition Of Fruit Tree Branches

Fruit tree branches not only occupy a large area of land and the burning of them will lead to the environmental pollution, but also it will waste the precious biological resources. This study separates and purifies the efficiency cellulose degradation strains from the product of the compositing and the soils samples in Qinling mountain area, and then fostering. Thus, they can be made to be the composite microbial agents and use them in the process of the fruits branch and pig manure composting.The changes of physico-chemical properties, enzyme activity, microbial community functional diversity and structural diversity in compost materials during fruit tree branches and pig manure composting were determined.1. Screening and identification of a cellulose-degrading microbial with high capacity and construction of communityBy the method of Congo-red staining to screen strains, and then to re-screen according to the enzyme activity of CMC and FPA in 6 days. Five strains that could decompose cellulose better were obtained from soil samples. Physiological and biochemical characteristics and molecular biology analysis demonstrated that 2-3,10-2,3-5,6-2 and 10-4 were Ralstoinia sp.,penicillium sp., penicillium Aurantioqriseum., penicillium aurantioqriseum., Acremonium alternatum and Chaetomium piluliferum, respectively. Then they were cultured by mixed fermentation. The combination of 10-2/3-5/2-3/6-2 enhanced cellulose containing CMC, FPA and Xylanase to some degree, and the activity of CMC, FPA and Xylanase was 16.3, 58.6 and 11083.7 U/m L, the study fond that the enzyme activity of the microbial community was higher than that from the fermentation of single bacteria.2. Effect of compound microbial agents inoculants on enzyme activity during fruiter branchesThe results showed the temperature during composting period was increased and high temperature(above 55 ℃) maintaining period was three days longer, the activity of the enzymes were improved by adding compound microbial agents. The tested enzyme Urease, Polyphenol oxidase, cellulose, Laccase(Lac), Manganese peroxidase(Mn P) and Lignin peroxidase(Li P) activities were higher in the treatment with microorganism agent than those in the control treatment by 2.3%~71.4%、0.3%~28.4%, 15.0%-19.8%, 1.0%-11.0%,4.1%-26.8% and 4.0%-22.2%, respectively.3. Effect of compound microbial agents inoculants on microbial community functional diversity during fruiter branchesThe AWCD value of microbes in compost was improved significantly and 6 kinds of carbon sources used by the microbes during the high temperature period and cooling period were also significantly changed by the inoculation compound microbial agents. Principal component analysis clearly revealed that adding compound microbial agents play mainly a role in the high temperature period during composting, and the differentiation in utilization of carbon sources were mainly sugars and amino acids.The results of testing sequencing show that in the process of the aerobic composting the advantages bacteria are mainly the Bacillus sp. PML14, Pseudomonas sp., Firmicutes bacterium, Pseudomonas sp., Phormidium sp and a large number of bacteria which cannot be fostered by the traditional methods but played an important role in the composting process, among these bacteria, Bacillus sp., Uncultured bacterium isolate DGGE gel band, Phormidium sp. And Uncultured beta proteobacterium isolate DGGE gel band played a main role in the high temperature of composting period. The process of the inoculants agent can significantly improve the diversity of the composting process of bacteria community, it can also promote the succession of the structure of bacteria and accelerate the process of compost rotten.