Preparation Of Gold Nanoparticles As Detection Probes And Application Of Detection Of Heavy Metal Ions In Aquatic Vegetables

Aquatic vegetables are more and more popular with consumers due to its rich nutrition value and health benefits of being both medicinal and edible, howerer with the continuous development of urban industrialization, the contamination of the water environment where aquatic vegetables grow is being worsen, especially pollution of heavy metal ions, heavy metal ions can hardly be metabolized and accumulate in the aquatic vegetables due to the rich vascular structure which can easily absorb metal ions, once ingested by the body they can do harm to the health of people, conventional detection methods of heavy metal ions are complex, cumbersome, expensive and need a long time, we establish a rapid, low cost, visualization methods based on gold nanoparticles to detect heavy metal ions green in this paper. Contents of this paper are as follows:1. We established the Au NPs-GSH-Na Cl detection system to detect Cu2+, using this method we can quickly and semi-quantitative detect Cu2+ based on the change of detection system color which can be visualized by naked eyes, when the concentration of Cu2+ in the sample is equal to or more than 0.04 mg/L, visible color change can be observed. The detection of Cu2+ can also be accurate quantitative, the linear range is from 0.01 mg/L to 0.1 mg/L, the lowest detection limit was 0.01 mg/L, and the methods was used in water chestnuts and lotus root samples to detect Cu2+, the results of spiked recovery rate of Cu2+ in two samples were 87.5% to 94.4%, indicating that the method of detecting Cu2+ in actual samples is feasible.2. We established a kind of method to detect Cr by gold nanoparticles modified glutathione, the method had good selectivity and high sensitivity, using this method can semi-quantitative detect Cr3+ by naked eye, when the concentration of Cr3+ in the sample is not less than 0.5 mg/L can be observed visible color change, The detection of Cr3+ can also be accurate quantitative, the linear range is from 0.1 mg/L to 1 mg/L, the lowest detection limit was 0.03 mg/L, and the methods was used in water chestnuts and lotus root samples for the testing of Cr3+, the results of spiked recovery rate of Cr3+ in two samples were 85% to 95.7%, indicating that the method is feasible in the detection of Cr3+ in real samples.3. We established a kind of method to detect Hg2+ by the gold clusters synthesised by bovine serum albumin as a template. The method had good selectivity and high sensitivity, this method can be used to detect Hg2+ in aquatic vegetable samples, the linear range is from 0.01 mg/L to 1 mg/L, the lowest detection limit of this method was 0.0095 mg/L, and the methods was used in water chestnuts and lotus root samples for the testing of Hg2+, the results of spiked recovery rate of Hg2+ in two samples were 87.6% to 106.9%, indicating that the method of detecting Hg2+ in actual samples was feasible.