Preparation Of Nanoporous Silica Membranes And Applied In Protein Molecular Imprinting

Current protein analysis is mostly based on antibody system. However, there are some drawbacks for antibody system, such as complicated preparation process, high cost and poor stability. In the present thesis, we have designed a protein imprinting method based on nanoporous materials, which combine the advantages of 3D protein imprinting(high capacity) and 2D protein imprinting(high mass transfer, easy integration with sensor platforms) together. The imprinting membranes have be prepared by using silica sol-gel technique and molecule-imprinting method. A stable, cheap and general protein separation/analysis system has been fabricated based on protein imprinting. There are three main research contents included in this thesis:1. Research on the properties of the membranes made by sol-gel technique, such as mechanical properties(hardness, modulus and brittleness), surface morphology and membrane thickness: We have studied the affect of sol components and aging time on its mechanical properties, it was found that when the mole ratio of DMDMOS to TMOS(Tetraethoxysilane) was 1:1, the mole ratio of H2 O to TMOS was 4:1, and the aging time was 16 h, the membranes had the better mechanical properties and more smooth surface morphology. We also investigated the effect of sol concentration on the film thickness. The membrane’s thickness from 160 nm to 1200 nm could be easily acquired by dilution with 2-propanol.2. The fabrication of transferrable self-supporting nanoporous silica membranes: Bacteria and polystyrene microspheres were used as templates to fabricate the porous silica membranes. The vertical deposition method and sol-gel method were combined to make large-scale self-supporting silica membranes with highly ordered, interconnected, 3D pore architectures. The diameters and number of layers of the 3D pore architectures can be manipulated by changing the microsphere diameter(200nm, 290 nm, 400nm) and concentration respectively.3. The fabrication of nanoporous membranes with protein recognition ability: the molecule imprinting method, the vertical deposition method and sol-gel method were combined to fabricate membranes with protein recognition ability. Cytochrome C was used as template molecule to prepare the protein molecularly imprinted membrane. We increased the membrane’s combining capacity and mass transfer ability by optimizing the density of polystyrene microspheres, p H of phosphate buffer solution and the layers of protein molecularly membrane.