Studies Of 4-Aminoantipyrine Molecular Imprinting Technique Based On Silica Gel As Sacrificial Materials

In this article, developments on synthetic method of molecular imprinting polymers (MIPs), especially the one based on the sacrificial material-silica-gel, are reviewed. And MIP's applications on chromatography separation, mimic antibody, solid-phase extraction and bio-mimetic sensors and so on are summarized in detail. In addition, the purpose an the design of the experiment is pointed out briefly.In the polymerization procedure, two methods are used. Buck radical polymerization is used to verify the sorts of the template, functional monomer, cross-linker and porogen, and optimize the amount of the porogen. This relatively better system is adopted as a pre-polymerization compound of the sacrificial silica-gel method. In the method, different paler-coated Silicas are used as sacrificial materials to study their influence on the absorption of the MIPs by a Ov-vis absorption measure techndogy.Factors which impact on the MIP's absorption ability are discussed ail-roundly, emphatically some interrelated prominent factors such as stabilization property of the complex; the reaction time's control in the polymerization; the ground strength the washing method and solvents, and the drying process after the polymerization; and the dissolution and swollen in the applications. The results of adsorption isotherms researches can obtain the saturation adsorption quantity of the MIPs. The results of the adsorption dynamics researches have qualitatively shown the adsorption rate of MIPs for template molecular, The dissociation constants and the apparent maximum binding quantities are calculated by scat chard analysis, and the concentration of solution has a great effect on the results of Seat chard analysis, the competitive binding experiment is used to investigate MIP's selective recognition to the template and its analogues. The effects of chemically functional-group on the separation and the contributions of ionic and hydrogen bond interactions to the separation were also investigated to reveal the mechasim of the molecular recognition process.The results showed that the MIPs which is prepared using non-polar silica-gel as a sacrificial material has the saturation adsorption quantity, the faster adsorption rate, and the higher recognition coefficient. All this were significant to prepare for MIP's application in purification and determination of Aminoantipyrine.