Dietary Exposure And Risk Assessment Of Deoxynivalenol In Shaanxi Wheat And Its Product

Deoxynivalenol(DON) is known as Vomitoxin which widely exist and seriously contaminate wheat. It has very strong virulent to human body, so that is easy to pose a threat to public health. Deoxynivalenol is a Trichothecenes produced by Fusarium Graminearum and Fusarium Roseum. Because Shaanxi province is one of the major wheat-growing regions, it is significant to assess the potential risk to public safety in DON of wheat. In this paper, we compared different standard method and used water extraction-monoclonal antibody immuneoaffinity column-HPLC to detect deoxynivalenol of Shaanxi wheat and then combine with demographic data to establish the model. After choosing different exposure assessment methods, the non parametric model uses Bootstrap and Bias-Corrected and Accelerated method to make exposure assessment in different economic regions, ages and sexes. The main results are as following:(1)Compared with Water Extraction-immune affinity column-HPLC detection(GB/T 23503-2009), Organic Solvent Extraction-immune affinity column-HPLC detection(SN/T 1571-2005), Solid-Phase Extraction column-HPLC(SN/T 3173-2012) standard methods. Through the comparison and analysis, Water Extraction-immune affinity column- HPLC method is of high recovery rate, high accuracy and good precision, that can be applied to large scale testing sample size vomitoxin.(2)Combined with tasting data of 201 samples of wheat in Shaanxi province and the consumption data and demographic data. We simulated point assessment, the parameters of probability assessment(traditional Monte Carlo model), the nonparametric probability evaluation model(the Bootstrap- BCa), and compared and analyzed the advantages and disadvantages. Nonparametric exposure assessment is suitable for processing complex models, especially the mycotoxins such trace samples nonparametric distribution, using the Bootstrap sampling method, combined with Bias- Corrected and Accelerated correction. Estimate vomitoxin daily intake in wheat with do dietary exposure assessment, analyze the variability and accuracy.(3)The result shows: the averages of all groups of people are lower than the Provisional Maximum Tolerable Daily Intake(1μg/kg bw/day) by JECFA; The intake of 2-4 year-old children is highest, and the old over 70 is lowest. The intake of adolescents is higher than in adults; Because of taking wheat products as staple food, the average of the third type in every groups is higher than other regions. There are three types rural areas with 2-4 year-old boys having 99 th percentile which is higher than PMTDI. In the long run, Shaanxi’s wheat makes low risk for public health, but food department should keep monitoring changes of DON in wheat, and some populations whose common food staples are wheat products should reduce the intake of DON.