Study On The Recombinant Lysostaphin Hydrocolloid Wound Dressing

Lysostaphin is an enzyme with a wide-spectrum antibacterial activity, which has been widely used in treatment of multi-bacterial infection. This research focus on the development of a hydrocolloid wound dressing with recombinant lysostaphin as main component. We chose polyvinyl alcohol(PVA), Carboxymethylcellulose sodium(CMC-Na) and gelatin as matrix to prepare several hydrocolloids and evaluated with liquid absorptivity, water vapor permeability, bacteria blocking ability and other methods. We found the optimal proportion of 5% PVA,3% CMC-Na and 10% gelatin, which has the highest stability and good repeatability. The release of the recombinant lysostaphin from the hydrocolloid in in vitro sustained release test of 2 hours has reached more than 80% of the labeled amount; in vitro sustained release test solution of 2 hours has bacteria killing rate of over 90%; the weight variation limit is within ±7.5%; microbial limit test results meet the requirements. The quality research methods have good reproducibility and can be used for quality control on sustained release and bacteria killing rate of the preparation; in addition the quality research confirms that the production process of recombinant lysostaphin hydrocolloid is stable and the product is a wound dressing with good antibacterial property. By studying the quality of the in vitro release degree, bacteria killing rate, weight variation, microbial limit of recombinant lysostaphin hydrocolloid, we established a quality standard of recombinant lysostaphin hydrocolloid wound dressing.