Method For Determination Of Citrinin In Food Material

Citrinin is a class of highly toxic mycotoxins produced in Penicillium, Aspergillus, Monascus metabolic processes in cereals, fruits, meat and related products have a presence.With the development of times,the contamination of citrinin in food become more and more serious.Citrinin will pile up in human body to increased health dangers. It’s very imminent to establish method for the citrinin testing in different kind of food.In this thesis,the method for the analysis of citrinin was researched.Liquid liquid extraction,dispersed solid phase extraction and ultrasonic extraction combined whith the application of fluorescence spectrophotometry, HPLC and capillary electrophoresis to detect citrinin in food.And it was proved effective by the experimental methodology,which concluded as followed:1、Comprehensive method for determining the content of citrinin in condiment was reported.The influence of the pH, solvent and wavelength on the stability of citrinin was investigated. The samples were extracted with 60%ethanol and enriched by rotary evaporator.The excitation and emission wave-lengths in the measurement were 300 nm,501 nm respectivelv.In this interval, the calibration curve is Y=20.31X+7.60, and R2 is 0.9922, RSD was 6.81%, the limit of detection is 0.03 ug/mg and the average recovery is 81.26%-90.40%. This method was simple and accurate, which can be used for deterting citrinin in condiment.2、To establish a method to determine the content of citrinin in grain.The samples were extracted with 60% ethanol, and cleaned up by dispersive solid-phase extraction with C18 dispersant sorbent and ready for HPLC determination.Results:A standard curve equation was built as follows:Y=3.13×10-7X-5.59×10-2 with linear correlation coefficient of 0.9999.RSD was 4.51%. The average recovery range was 87.7%-96.4%.The citrinin content range of the samples was 0.116～0.385ug/g.This method was sample and accurate,which could be used for detecting citrinin in grain.3、The samples were extracted with ethanol, pretreated by ultrasonic extraction in the condition of 175W,30℃,40min.And ready for CE determination.Meanwhile a running voltage 15 kV,20 mmol·L-1 borax buffer(pH 9.0) and a UV detector at 251 nm were adopted.A standard curve equation was built as follows:Y=721.52X+672.93 with linear correlation coefficient of 0.9953.The limit of detection is 2.0 ug/mL.and the calibration graphs were rectilinear from 2.0-50 ug/mL.This method was sample and accurate,which could be used for detecting citrinin in fruits.