Research On Application Of Amino Acid In Silk’s Aging Degree Evaluation

The existing historical silk is an indispensable physical carrier for the exploration andresearch of ancient civilization, it contains great value about history and academic research.Unearthed silk fabrics degradated in varying degrees because of a combination of various factorsin the process of burial and excavation. Appropriate methods is important to protect and repairthe silk relics. We need to select them after scientific study and analyze based on the principle ofnondestructive. As a detection technology based on the molecular level,amino acid detectingtechnology attracts so much attention for its trace amounts of test samples.In this paper we made artificial aging samples and testing their basic feature such asapparent properties, mechanical performance, infrared spectroscopy to preliminarily realized theaging degree. Analyze the different reactions of several characteristic amino acid of agingsamples in different aging conditions(lighting, thermal, hydrolyzation) by pre-columnderivatization and reserved-phase high performance liquid chromatography(RP-HPLC)fluorescence detection. The result showed that amino acids have different degree of loss with theextending of aging degree. Tyrosine is sensitive to light and thermal aging. On the other hand,aspartic acid and glutamic acid are more sensitive to water testing, therefore it characterized thehydrolysed aging degree better.Think of the percentage of amino acid with bulky-side as ameasure of ageing degree, We classifying six kinds of amino acids (glycine, alanine, serine andthreonine, proline, histidine) as a nonbulky-side group and the rest of remained as bulky-sidegroup amino acids. The research treated the bulk-side group as a measurement to gauge silksamples’ degree, and the data processing result also forming a good linear relationship with thetime of aging.Through exploring the relationship between amino acid data and force retention, Fourierinfrared spectrum test results, it can be found that the relationship between the percentage ofamino acid with bulk-side group and the rest of the performance parameters formed goodcorresponding relation. And the data of amino acid with bulk-side groups’ percentage is morecomprehensive and effective than single amino acid. Thus, the data statistics system about aminoacid with bulk-side group-breaking strength retention rate relationship established, and it can beevaluating degradation degree of silk fabrics as the evaluation system. The research tested theamino acids of silk relics from three tombs: Anji Wufu Chu tomb, Xinjiang Yingpan Han tomband Jiangxi Nanchang Ming tomb. The results showed that it has certain feasibility to estimate the degradation degree of silk relics sample by the way to study its position in the statisticaldiagram position. The destructive degree estimate results of samples from the three tombs withthis method are consistent with the results of the cross section morphology observation of thesample.In other words, the relics sample analysis results verified that the percentage ofbulky-side amino acid having a certain reference on the relics sample degradation assessment.