Drug Carrier Preparation And Inorganic Nanomaterial Modification Based On Functionalized Poly(Amino Acid)

Biocompatible polymers have been deeply studied as drug carriers and man-made biomaterials while functionalized biocompatible are used more widely. For the hydrophobic drug carriers and hydrophobic nanoparticle surface modification, hydrophobic parts of the amphiphilic polymer were highly desired. Through hydrophobic interaction, the amphiphilic polymers are able to self-assemble into a variety of nanostructures, improving drug delivery and medical diagnostics in vivo.This article focuses on the targeting delivery of the cocktail drug nanocapsules with pH response based on the biocompatible poly(amino acid). Meanwhile, we developed a versatile method for the preparation of inorganic-organic nanocomposite combined by silica and poly(amino acid) coating.The details are summarized as follows:1、We design a pH-responsive cocktail nanocapsule with suitable size (around 100 nm) via the assembly of doxorubicin (DOX) modified poly(amino acid). Paclitaxel (PTX) is successfully encapsulated in the hydrophobic cavity of nanocapsules through non-covalent interaction between PTX and DOX modified poly(amino acid). Guided by the surface bioconjugated Arg-Gly-Asp (RGD) moieties,a target peptide, nanocapsules are targeted to and uptaken by cancer cells.These nanocapsules are stable under normal physiological pH conditions (pH 7.4) and the drug release can be triggered by the relatively low pH (5.0) in cancer cells. Moreover, the drug release can be tracked via the recovered red fluorescence of DOX due to the relieving from self-quenching state as the result of nanocapsule decomposition under acidic conditions. This research paves a new way for the fabrication of smart nanocapsules for cocktail drug delivery.2、To simplify the preparation of silica-coated inorganic functional nancomposite, we have developed method for nanocomposite based on poly(amino acid) and silica coating, in which octadecyltrimethylammoniun as a silane source. The formation of silica-coated functional inorganic nanocomposite was assisted by ultrasonication. Oleylamine modified polysuccinimide provides a stable hydrophobic cavity, providing more space for the hydrophobic inorganic nanoparticles. Octadecyl trimethoxy silane has a hydrophobic end to interact with the nanoparticles while the other end condenses through hydrolyzation in an alkaline, forming biocompatible silica layer. The thickness of the silica layer can be adjusted by the TEOS dosage. After subsequent alkali treatment, we obtain a high nanoparticle loading, uniform morphology silica-coated functional inorganic nanocomposite with potentials in bioimaging, drug delivery and catalysis.